Cat: PAX2000-10687

Recombinant Human RAB33A Protein,His

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Analytical Data

  • Gene name

    RAB33A

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    Small GTP-binding protein S10

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q14088

  • Expression Region

    1-237 aa

  • AA Sequence

    MAQPILGHGSLQPASAAGLASLELDSSLDQYVQIRIFKIIVIGDSNVGKTCLTFRFCGGTFPDKTEATIGVDFREKTVEIEGEKIKVQVWDTAGQERFRKSMVEHYYRNVHAVVFVYDVTKMTSFTNLKMWIQECNGHAVPPLVPKVLVGNKCDLREQIQVPSNLALKFADAHNMLLFETSAKDPKESQNVESIFMCLACRLKAQKSLLYRDAERQQGKVQKLEFPQEANSKTSCPC

  • Molecular Weight

    34.1 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

RAB33A is a member of the RAB family of small GTPases, which are critical regulators of intracellular vesicular trafficking. Research on RAB33A has gained attention due to its involvement in various cellular processes, including autophagy, exocytosis, and endocytosis. Emerging studies suggest that RAB33A plays a significant role in the biogenesis of autophagosomes and the regulation of Golgi apparatus functions. Its abnormal regulation has been implicated in several diseases, including neurodegenerative disorders and cancers. Therefore, the production of recombinant RAB33A protein is essential for elucidating its molecular mechanisms and functional roles. By utilizing recombinant DNA technology, researchers can generate large quantities of RAB33A for in vitro studies, enabling them to investigate its interactions with effector proteins and its impact on cellular pathways. Understanding RAB33A's function at the structural and molecular levels could reveal potential therapeutic targets for diseases linked to its dysregulation, thereby advancing our knowledge of cellular dynamics and disease mechanisms.

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