Analytical Data
-
Gene name
CA9
- Application
-
Alternative Names
CA9；Collagen alpha-1(IX) chain
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q16790
-
Expression Region
59-414aa
-
AA Sequence
PLGEEDLPSEEDSPREEDPPGEEDLPGEEDLPGEEDLPEVKPKSEEEGSL KLEDLPTVEAPGDPQEPQNNAHRDKEGDDQSHWRYGGDPPWPRVSPACAG RFQSPVDIRPQLAAFCPALRPLELLGFQLPPLPELRLRNNGHSVQLTLPP GLEMALGPGREYRALQLHLHWGAAGRPGSEHTVEGHRFPAEIHVVHLSTA FARVDEALGRPGGLAVLAAFLEEGPEENSAYEQLLSRLEEIAEEGSETQV PGLDISALLPSDFSRYFQYEGSLTTPPCAQGVIWTVFNQTVMLSAKQLHT LSDTLWGPGDSRLQLNFRATQPLNGRVIEASFPAGVDSSPRAAEPVQLNS CLAAGD
-
Molecular Weight
39 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Research on CA9 (Carbonic Anhydrase IX) recombinant proteins has gained significant attention due to the enzyme's critical role in cancer biology and its potential as a therapeutic target. CA9 is a membrane-bound protein that is frequently overexpressed in various tumors, particularly in renal cell carcinoma, making it a promising biomarker for cancer diagnosis and prognosis. The enzyme facilitates the conversion of carbon dioxide to bicarbonate and protons, playing a vital role in maintaining acid-base balance and promoting tumor growth in hypoxic conditions. Researchers have been focusing on the recombinant expression of CA9 to better understand its structure-function relationships, validate its role in tumor microenvironments, and explore its interactions with signaling pathways that contribute to cancer progression. Utilizing recombinant CA9 proteins allows for detailed studies of enzymatic activity, inhibitor screening, and the development of targeted therapies, including immunotherapies and small molecule inhibitors. Furthermore, the production of CA9 in a recombinant system enables high yield and purity, facilitating the study of its physiological and pathological roles. The ongoing exploration of CA9 not only enhances our understanding of cancer metabolism but also holds promise for innovative therapeutic strategies aimed at exploiting the vulnerabilities associated with CA9 overexpression in various malignancies.
