Analytical Data
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Gene name
PMEL
- Application
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Alternative Names
PMEL；D12S53E；PMEL17；SILV；Melanocyte Protein PMEL
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P40967
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Expression Region
1-661aa
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AA Sequence
MDLVLKRCLLHLAVIGALLAVGATKVPRNQDWLGVSRQLRTKAWNRQLYP EWTEAQRLDCWRGGQVSLKVSNDGPTLIGANASFSIALNFPGSQKVLPDG QVIWVNNTIINGSQVWGGQPVYPQETDDACIFPDGGPCPSGSWSQKRSFV YVWKTWGQYWQVLGGPVSGLSIGTGRAMLGTHTMEVTVYHRRGSRSYVPL AHSSSAFTITDQVPFSVSVSQLRALDGGNKHFLRNQPLTFALQLHDPSGY LAEADLSYTWDFGDSSGTLISRALVVTHTYLEPGPVTAQVVLQAAIPLTS CGSSPVPGTTDGHRPTAEAPNTTAGQVPTTEVVGTTPGQAPTAEPSGTTS VQVPTTEVISTAPVQMPTAESTGMTPEKVPVSEVMGTTLAEMSTPEATGM TPAEVSIVVLSGTTAAQVTTTEWVETTARELPIPEPEGPDASSIMSTESI TGSLGPLLDGTATLRLVKRQVPLDCVLYRYGSFSVTLDIVQGIESAEILQ AVPSGEGDAFELTVSCQGGLPKEACMEISSPGCQPPAQRLCQPVLPSPAC QLVLHQILKGGSGTYCLNVSLADTNSLAVVSTQLIMPGQEAGLGQVPLIV GILLVLMAVVLASLIYRRRLMKQDFSVPQLPHSSSHWLRLPRIFCSCPIG ENSPLLSGQQV
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Molecular Weight
97 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PMEL (Premelanosome Protein) is a crucial protein implicated in the formation of melanosomes, which are organelles responsible for the synthesis and storage of melanin in melanocytes. Understanding PMEL and its role in melanin metabolism is of significant interest not only for basic research in cell biology but also for clinical implications in disorders related to pigmentation, such as vitiligo, albinism, and melanoma. PMEL undergoes a complex series of post-translational modifications, leading to its oligomerization and subsequent incorporation into melanosomes. These processes are vital for the proper development of melanin pigmentation, and any disruption can lead to abnormal skin or hair pigmentation. Recent studies have utilized recombinant DNA technology to express and purify PMEL proteins, enabling detailed studies of their structural and functional properties. This innovative approach allows researchers to dissect the molecular mechanisms underlying PMEL’s role in melanin synthesis, paving the way for potential therapeutic strategies to address pigmentation disorders and enhance our understanding of cellular physiology associated with melanosome biogenesis and function. Thus, research on PMEL restructuring through recombinant protein methodologies represents a promising frontier in both basic science and translational medicine, enhancing our understanding of pigmentation and its associated pathologies.
