Analytical Data
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Gene name
MECP2
- Application
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Alternative Names
MECP2；Methyl-CpG-binding Protein 2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P51608
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Expression Region
1-486aa
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AA Sequence
MVAGMLGLRE EKSEDQDLQG LKDKPLKFKK VKKDKKEEKE GKHEPVQPSA HHSAEPAEAG KAETSEGSGS APAVPEASAS PKQRRSIIRD RGPMYDDPTL PEGWTRKLKQ RKSGRSAGKY DVYLINPQGK AFRSKVELIA YFEKVGDTSL DPNDFDFTVT GRGSPSRREQ KPPKKPKSPK APGTGRGRGR PKGSGTTRPK AATSEGVQVK RVLEKSPGKL LVKMPFQTSP GGKAEGGGAT TSTQVMVIKR PGRKRKAEAD PQAIPKKRGR KPGSVVAAAA AEAKKKAVKE SSIRSVQETV LPIKKRKTRE TVSIEVKEVV KPLLVSTLGE KSGKGLKTCK SPGRKSKESS PKGRSSSASS PPKKEHHHHH HHSESPKAPV PLLPPLPPPP PEPESSEDPT SPPEPQDLSS SVCKEEKMPR GGSLESDGCP KEPAKTQPAV ATAATAAEKY KHRGEGERKD IVSSSMPRPN REEPVDSRTP VTERVS
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MECP2 (methyl-CpG binding protein 2) is a crucial protein that plays a significant role in the regulation of gene expression and is essential for neuronal function and development. Mutations in the MECP2 gene are primarily associated with Rett Syndrome, a neurodevelopmental disorder predominantly affecting females, leading to severe cognitive and physical impairments. Research into MECP2 has gained momentum due to its pivotal role in neuronal maturation and synaptic plasticity. Understanding the structure and function of MECP2 is vital for elucidating its pathogenic mechanisms and for developing potential therapeutic strategies. Studies involving the recombinant expression of MECP2 offer insights into its biochemical properties and interactions with DNA, as well as its influence on chromatin structure. Through techniques like crystallography and biophysical analyses, researchers aim to identify how various mutations impact the protein's function. Additionally, investigations into MECP2's epigenetic roles highlight its importance in maintaining neuronal integrity and the potential for targeted interventions in MECP2-related disorders. Overall, the exploration of recombinant MECP2 not only enhances our comprehension of molecular neurobiology but also paves the way for innovative treatments for diseases associated with MECP2 dysregulation.
