Analytical Data
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Gene name
PFKFB1
- Application
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Alternative Names
PFKFB1；F6PK；PFRX；6-phosphofructo-2-kinase/fructose-2.6-bisphosphatase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P16118
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Expression Region
2-471aa
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AA Sequence
SPEMGELTQ TRLQKIWIPH SSGSSRLQRR RGSSIPQFTN SPTMVIMVGL PARGKTYIST KLTRYLNWIG TPTKVFNLGQ YRREAVSYKN YEFFLPDNME ALQIRKQCAL AALKDVHNYL SHEEGHVAVF DATNTTRERR SLILQFAKEH GYKVFFIESI CNDPGIIAEN IRQVKLGSPD YIDCDREKVL EDFLKRIECY EVNYQPLDEE LDSHLSYIKI FDVGTRYMVN RVQDHIQSRT VYYLMNIHVT PRSIYLCRHG ESELNIRGRI GGDSGLSVRG KQYAYALANF IQSQGISSLK VWTSHMKRTI QTAEALGVPY EQWKALNEID AGVCEEMTYE EIQEHYPEEF ALRDQDKYRY RYPKGESYED LVQRLEPVIM ELERQENVLV ICHQAVMRCL LAYFLDKSSD ELPYLKCPLH TVLKLTPVAY GCKVESIYLN VEAVNTHREK PENVDITREP EEALDTVPAH Y
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PFKFB1 (Phosphofructokinase 2/fructose bisphosphatase 2) is a key regulatory enzyme in the glycolytic pathway, playing a crucial role in controlling glucose metabolism. It synthesizes fructose 2,6-bisphosphate, a potent allosteric activator of phosphofructokinase-1 (PFK-1), thereby stimulating glycolysis while inhibiting gluconeogenesis. Dysregulation of PFKFB1 has been implicated in various metabolic diseases, including cancer, diabetes, and obesity, where altered glycolytic flux promotes cell survival and proliferation. Given its significant role in metabolic control and disease pathogenesis, recombinant PFKFB1 protein has garnered attention for understanding its structure-function relationships, catalytic mechanism, and regulatory pathways. Research involving this recombinant protein can elucidate the molecular basis of its activity and regulation, offering insights into potential therapeutic targets for metabolic disorders. Additionally, PFKFB1 serves as a model for studying enzymatic regulation under different physiological and pathological conditions, making it a valuable protein for both basic and applied research. Through recombinant technology, researchers can produce PFKFB1 in sufficient quantities to facilitate kinetic studies, inhibitor screening, and exploration of its interactions with other metabolic regulators. This research not only enhances our understanding of metabolic regulation but also contributes to the development of novel strategies for managing diseases associated with glucose metabolism imbalance.
