Analytical Data
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基因名
KARS
- Application
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别名
KARS；KARS；KIAA0070；Lysine--tRNA ligase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q15046
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表达区间
63-597aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMGSGVGPEEESVDPNQYYKIRSQAIHQLKV NGEDPYPHKFHVDISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASG GKLIFYDLRGEGVKLQVMANSRNYKSEEEFIHINNKLRRGDIIGVQGNPG KTKKGELSIIPYEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDF VRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNE LDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCE FYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTP PFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRT TARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELF VMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEY GLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLE STTVGTSV
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分子量
64 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KARS, or lysyl-tRNA synthetase, is an essential enzyme that plays a critical role in protein synthesis by catalyzing the attachment of lysine to its corresponding tRNA molecule. The study of KARS has gained significant attention due to its involvement in various biological functions beyond its enzymatic activity, including its role in cellular signaling and regulation. Recent research has revealed that KARS can participate in processes such as angiogenesis, inflammation, and cancer progression, making it a potential target for therapeutic intervention. Moreover, the unique structural characteristics of KARS allow for the exploration of its function and regulation at a molecular level, providing insights into the mechanisms of translation and aminoacylation. The reorganization of KARS into recombinant proteins opens up new avenues for detailed studies, enabling scientists to investigate its structure-function relationships, interactions with other biomolecules, and potential applications in biotechnology and medicine. Given its multifaceted roles, the research on KARS and its recombinant forms is not only significant for understanding fundamental biological processes but also for developing novel strategies for disease treatment and management. Thus, the exploration of KARS presents a promising frontier in molecular biology and therapeutics, highlighting its importance as a subject of intensive study.
