Analytical Data
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Gene name
NDST1
- Application
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Alternative Names
NDST1；HSST；HSST1；Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P52848
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Expression Region
40-556aa
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AA Sequence
GWKRGLEPSADAPEPDCGDPPPVAPSRLLPLKPVQAATPSRTDPLVLVFVESLYSQLGQEVVAILESSRFKYRTEIAPGKGDMPTLTDKGRGRFALIIYENILKYVNLDAWNRELLDKYCVAYGVGIIGFFKANENSLLSAQLKGFPLFLHSNLGLKDCSINPKSPLLYVTRPSEVEKGVLPGEDWTVFQSNHSTYEPVLLAKTRSSESIPHLGADAGLHAALHATVVQDLGLHDGIQRVLFGNNLNFWLHKLVFVDAVAFLTGKRLSLPLDRYILVDIDDIFVGKEGTRMKVEDVKALFDTQNELRAHIPNFTFNLGYSGKFFHTGTNAEDAGDDLLLSYVKEFWWFPHMWSHMQPHLFHNQSVLAEQMALNKKFAVEHGIPTDMGYAVAPHHSGVYPVHVQLYEAWKQVWSIRVTSTEEYPHLKPARYRRGFIHNGIMVLPRQTCGLFTHTIFYNEYPGGSSELDKIINGGELFLTVLLNPVSAPQPMAAGEKGLLHSLSAADTGFLEPGKGGEA
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Molecular Weight
73.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NDST1 (N-deacetylase/N-sulfotransferase 1) is an enzyme crucial for heparan sulfate biosynthesis, playing a significant role in various biological processes including cell signaling, development, and tissue repair. Its activity involves the modification of heparan sulfate proteoglycans, which are essential for mediating interactions between cells and extracellular molecules. Dysregulation of NDST1 has been implicated in several diseases, including cancers and genetic disorders. Research into NDST1 recombinant protein aims to elucidate its mechanisms of action and potential therapeutic applications. By producing and studying NDST1 in a recombinant form, scientists can investigate its structure-function relationship and the impact of specific mutations or modifications. Understanding NDST1's role could pave the way for novel strategies in targeting diseases associated with its malfunction, thus providing essential insights into cell signaling pathways and opportunities for drug development. Overall, the exploration of NDST1 and its recombinant protein holds promise for advancing both fundamental biology and clinical applications in disease treatment.
