Cat: PA2000-2115

Recombinant Human NAPRT Protein,His

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Analytical Data

  • Gene name

    NAPRT

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    NAPRT;FHIP;NAPRT1;Nicotinate phosphoribosyltransferase

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q6XQN6

  • Expression Region

    229-538aa

  • AA Sequence

    MLAPAAGEGPGVDLAAKAQVWLEQVCAHLGLGVQEPHPGERAAFVAYALAFPRAFQGLLDTYSVWRSGLPNFLAVALALGELGYRAVGVRLDSGDLLQQAQEIRKVFRAAAAQFQVPWLESVLIVVSNNIDEEALARLAQEGSEVNVIGIGTSVVTCPQQPSLGGVYKLVAVGGQPRMKLTEDPEKQTLPGSKAAFRLLGSDGSPLMDMLQLAEEPVPQAGQELRVWPPGAQEPCTVRPAQVEPLLRLCLQQGQLCEPLPSLAESRALAQLSLSRLSPEHRRLRSPAQYQVVLSERLQALVNSLCAGQSP

  • Molecular Weight

    35.2 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

NAPRT (Nicotinate Adenine Dinucleotide Phosphate Regenerator) is a key enzyme involved in the NAD+ biosynthetic pathway, specifically catalyzing the conversion of nicotinate to NAD+, an essential cofactor in various metabolic processes. Research into NAPRT and its recombinant proteins has gained momentum due to its significance in cellular metabolism and energy production, making it a potential target for therapeutic applications. Disruption of NAD+ levels has been implicated in various diseases, including metabolic disorders, neurodegenerative diseases, and cancer. Therefore, understanding the structure, function, and regulatory mechanisms of NAPRT can provide valuable insights into its role in these conditions. Additionally, recombinant NAPRT proteins can serve as valuable tools for studying NAD+ metabolism and developing novel strategies for drug discovery. The exploration of NAPRT’s enzymatic properties and its interactions with other cellular components can pave the way for innovative approaches to modulate NAD+ levels in diseased states, thus highlighting the importance of NAPRT in both basic research and potential clinical applications.

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