Analytical Data
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基因名
POLD2
- Application
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别名
POLD2；DNA polymerase delta subunit 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P49005
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表达区间
1-469aa
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氨基酸序列
MFSEQAAQRA HTLLSPPSAN NATFARVPVA TYTNSSQPFR LGERSFSRQY AHIYATRLIQ MRPFLENRAQ QHWGSGVGVK KLCELQPEEK CCVVGTLFKA MPLQPSILRE VSEEHNLLPQ PPRSKYIHPD DELVLEDELQ RIKLKGTIDV SKLVTGTVLA VFGSVRDDGK FLVEDYCFAD LAPQKPAPPL DTDRFVLLVS GLGLGGGGGE SLLGTQLLVD VVTGQLGDEG EQCSAAHVSR VILAGNLLSH STQSRDSINK AKYLTKKTQA ASVEAVKMLD EILLQLSASV PVDVMPGEFD PTNYTLPQQP LHPCMFPLAT AYSTLQLVTN PYQATIDGVR FLGTSGQNVS DIFRYSSMED HLEILEWTLR VRHISPTAPD TLGCYPFYKT DPFIFPECPH VYFCGNTPSF GSKIIRGPED QTVLLVTVPD FSATQTACLV NLRSLACQPI SFSGFGAEDD DLGGLGLGP
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POLD2, or DNA polymerase delta 2, is a crucial component of the DNA replication machinery in eukaryotic cells, playing an essential role in DNA synthesis and repair. It is part of the larger DNA polymerase delta complex, which is responsible for lagging strand synthesis during DNA replication and contributes to maintaining genomic stability by participating in DNA repair processes. Research on the recombinant POLD2 protein has gained significant interest due to its implications in cancer biology and genetic disorders. Aberrant activity or mutations in POLD2 can lead to genomic instability, a hallmark of cancer cells, thereby making it a potential target for cancer therapies. Moreover, the study of POLD2 in a recombinant form allows for detailed examination of its enzymatic properties and interaction with other protein partners involved in the replication and repair pathways. Investigating POLD2's structure-function relationships can reveal critical insights into how its dysfunction contributes to diseases, providing avenues for developing diagnostic and therapeutic strategies. Overall, recombinant POLD2 protein research is vital for understanding the fundamental mechanisms of DNA replication and repair, with far-reaching implications for cancer treatments and the understanding of hereditary conditions related to DNA repair deficiencies. Such studies not only enhance our comprehension of the molecular underpinnings of cell proliferation and genome integrity but also open doors for innovative therapeutic approaches targeting DNA polymerase activities in various malignancies.
