Analytical Data
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Gene name
GRM5
- Application
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Alternative Names
GRM5；GPRC1E；MGLUR5；Metabotropic glutamate receptor 5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P41594
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Expression Region
22-580aa
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AA Sequence
SSERRVVAHMPGDIIIGALFSVHHQPTVDKVHERKCGAVREQYGIQRVEAMLHTLERINSDPTLLPNITLGCEIRDSCWHSAVALEQSIEFIRDSLISSEEEEGLVRCVDGSSSSFRSKKPIVGVIGPGSSSVAIQVQNLLQLFNIPQIAYSATSMDLSDKTLFKYFMRVVPSDAQQARAMVDIVKRYNWTYVSAVHTEGNYGESGMEAFKDMSAKEGICIAHSYKIYSNAGEQSFDKLLKKLTSHLPKARVVACFCEGMTVRGLLMAMRRLGLAGEFLLLGSDGWADRYDVTDGYQREAVGGITIKLQSPDVKWFDDYYLKLRPETNHRNPWFQEFWQHRFQCRLEGFPQENSKYNKTCNSSLTLKTHHVQDSKMGFVINAIYSMAYGLHNMQMSLCPGYAGLCDAMKPIDGRKLLESLMKTNFTGVSGDTILFDENGDSPGRYEIMNFKEMGKDYFDYINVGSWDNGELKMDDDEVWSKKSNIIRSVCSEPCEKGQIKVIRKGEVSCCWTCTPCKENEYVFDEYTCKACQLGSWPTDDLTGCDLIPVQYLRWGDPEP
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Molecular Weight
70.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The metabotropic glutamate receptor 5 (GRM5) is a G protein-coupled receptor that plays a crucial role in synaptic transmission and plasticity in the central nervous system. Its dysregulation has been implicated in various neurological and psychiatric disorders, including autism, schizophrenia, and anxiety. As a result, GRM5 has emerged as a target for drug development aimed at treating these conditions. To better understand the receptor's structure, function, and pharmacology, researchers often produce recombinant GRM5 proteins. These proteins are crucial for elucidating the receptor’s signaling pathways and interactions with other cellular components. By studying the recombinant GRM5, scientists can perform binding assays, structural analyses, and functional assays to explore its biophysical properties and potential as a therapeutic target. Recent advancements in protein engineering and cryo-electron microscopy have facilitated the high-resolution study of GRM5, allowing for the identification of binding sites for novel compounds. A deeper understanding of GRM5's mechanisms may provide insights into the pathophysiology of associated disorders and aid in the discovery of new interventions that could improve treatment outcomes. Therefore, the study of GRM5 recombinant proteins is a critical step in neuroscience research, bridging basic science with potential clinical applications.
