Analytical Data
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Gene name
FBXW4
- Application
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Alternative Names
FBXW4；FBW4；SHFM3；F-box/WD repeat-containing Protein 4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P57775
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Expression Region
1-412aa
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AA Sequence
MAAAAGEEEEEEEAARESAARPAAGPALWRLPEELLLLICSYLDMRALGRLAQVCRWLRRFTSCDLLWRRIARASLNSGFTRLGTDLMTSVPVKERVKVSQNWRLGRCREGILLKWRCSQMPWMQLEDDSLYISQANFILAYQFRPDGASLNRRPLGVFAGHDEDVCHFVLANSHIVSAGGDGKIGIHKIHSTFTVKYSAHEQEVNCVDCKGGIIVSGSRDRTAKVWPLASGRLGQCLHTIQTEDRVWSIAISPLLSSFVTGTACCGHFSPLRIWDLNSGQLMTHLGSDFPPGAGVLDVMYESPFTLLSCGYDTYVRYWDLRTSVRKCVMEWEEPHDSTLYCLQTDGNHLLATGSSYYGVVRLWDRRQRACLHAFPLTSTPLSSPVYCLRLTTKHLYAALSYNLHVLDFQNP
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Molecular Weight
62.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
FBXW4 is a member of the F-box protein family, which plays a critical role in the ubiquitin-proteasome degradation pathway, involved in the regulation of various cellular processes such as cell cycle progression, DNA repair, and signal transduction. The research surrounding FBXW4 has gained momentum due to its potential implications in cancer biology, particularly in how it may influence tumorigenesis through its regulatory effects on oncogenic and tumor suppressor proteins. Abnormal expression or mutations in FBXW4 can lead to dysregulation in protein degradation, contributing to uncontrolled cell proliferation and resistance to apoptosis, which are hallmarks of cancer. Additionally, FBXW4 has been implicated in other diseases, including cardiovascular and neurodegenerative disorders, highlighting its significance beyond cancer. Investigating the structure and function of the FBXW4 recombinant protein is crucial for understanding its biological roles and interactions at the molecular level. This could pave the way for novel therapeutic strategies targeting FBXW4 pathways, providing a potential avenue for treatment and better management of various diseases associated with its dysregulation. The ongoing studies aim to elucidate the mechanisms by which FBXW4 exerts its effects within cells, further shedding light on its biological importance and the prospect of developing targeted interventions.
