Cat: PA2000-1977

Recombinant Human ELANE Protein,His

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Analytical Data

  • Gene name

    ELANE

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    ELANE;ELA2;Neutrophil elastase

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P08246

  • Expression Region

    30-267aa

  • AA Sequence

    IVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH

  • Molecular Weight

    25.7 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

ELANE, or elastase, is an enzyme primarily produced by neutrophils and plays a crucial role in the immune response by degrading extracellular matrix components and modulating inflammation. Dysfunctional ELANE is linked to various pathological conditions, including congenital neutropenia and chronic inflammatory diseases. Research on recombinant ELANE proteins has gained momentum in recent years due to their potential therapeutic applications. By producing ELANE in a recombinant form, researchers can study its structure-function relationships, mechanisms of action, and the effects of specific mutations that could influence its activity. Additionally, recombinant ELANE can be engineered for enhanced stability, specificity, and efficacy in clinical applications. Such advancements could pave the way for new treatments aimed at restoring normal immune function in patients suffering from ELANE-related disorders or for developing novel therapeutics that harness the enzyme’s properties to target pathological processes effectively. Overall, the study of recombinant ELANE holds promise not only for understanding its biological significance but also for translating these insights into innovative therapeutic strategies.

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