Analytical Data
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Gene name
PRKAR1B
- Application
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Alternative Names
cAMP dependent protein kinase type I beta regulatory subunit; cAMP-dependent protein kinase type I-beta regulatory subunit; KAP1_HUMAN; PKARI beta; PRKAR 1; PRKAR 1B; PRKAR1; Prkar1b; PRKAR1B protein; Protein kinase cAMP dependent regulatory type I beta; RIbeta
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P31321
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Expression Region
2-381 aa
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AA Sequence
ASPPACPSE EDESLKGCEL YVQLHGIQQV LKDCIVHLCI SKPERPMKFL REHFEKLEKE ENRQILARQK SNSQSDSHDE EVSPTPPNPV VKARRRRGGV SAEVYTEEDA VSYVRKVIPK DYKTMTALAK AISKNVLFAH LDDNERSDIF DAMFPVTHIA GETVIQQGNE GDNFYVVDQG EVDVYVNGEW VTNISEGGSF GELALIYGTP RAATVKAKTD LKLWGIDRDS YRRILMGSTL RKRKMYEEFL SKVSILESLE KWERLTVADA LEPVQFEDGE KIVVQGEPGD DFYIITEGTA SVLQRRSPNE EYVEVGRLGP SDYFGEIALL LNRPRAATVV ARGPLKCVKL DRPRFERVLG PCSEILKRNI QRYNSFISLT V
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Molecular Weight
43.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PRKAR1B, a gene encoding the type I regulatory subunit of protein kinase A (PKA), plays a crucial role in various cellular processes, including metabolism, gene expression, and cell proliferation. Dysregulation or mutations in this gene have been linked to several pathologies, including cardiac dysfunction, cancer, and endocrine disorders. The study of PRKAR1B recombinant proteins is particularly significant as these proteins can provide insights into PKA signaling pathways and their implications in health and disease. By producing PRKAR1B in recombinant systems, researchers aim to investigate its structural and functional properties, enabling a deeper understanding of how variations in this subunit influence PKA activity and downstream signaling cascades. This research could potentially lead to the identification of novel therapeutic targets and strategies for diseases associated with PKA dysregulation. The recombinant PRKAR1B proteins serve as valuable tools for biochemical assays, structural analysis, and drug discovery, advancing the field of cellular signaling and therapeutics.
