Analytical Data
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Gene name
PRIM2A
- Application
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Alternative Names
PRIM2; PRIM2A; DNA primase large subunit; DNA primase 58 kDa subunit; p58
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P49643
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Expression Region
1-509 aa
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AA Sequence
MEFSGRKWRK LRLAGDQRNA SYPHCLQFYL QPPSENISLI EFENLAIDRV KLLKSVENLG VSYVKGTEQY QSKLESELRK LKFSYRENLE DEYEPRRRDH ISHFILRLAY CQSEELRRWF IQQEMDLLRF RFSILPKDKI QDFLKDSQLQ FEAISDEEKT LREQEIVASS PSLSGLKLGF ESIYKIPFAD ALDLFRGRKV YLEDGFAYVP LKDIVAIILN EFRAKLSKAL ALTARSLPAV QSDERLQPLL NHLSHSYTGQ DYSTQGNVGK ISLDQIDLLS TKSFPPCMRQ LHKALRENHH LRHGGRMQYG LFLKGIGLTL EQALQFWKQE FIKGKMDPDK FDKGYSYNIR HSFGKEGKRT DYTPFSCLKI ILSNPPSQGD YHGCPFRHSD PELLKQKLQS YKISPGGISQ ILDLVKGTHY QVACQKYFEM IHNVDDCGFS LNHPNQFFCE SQRILNGGKD IKKEPIQPET PQPKPSVQKT KDASSALASL NSSLEMDMEG LEDYFSEDS
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Molecular Weight
58.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PRIM2A, or Primase polyprotein 2A, plays a crucial role in the process of DNA replication and repair, particularly in the context of the eukaryotic cellular machinery. This protein is essential for synthesizing short RNA primers, which are necessary for DNA polymerase to initiate the synthesis of new DNA strands. Given the significance of efficient DNA replication in maintaining genomic stability, studying PRIM2A has garnered attention in molecular biology and biotechnology. Mutations or dysregulation in primase activities can lead to severe consequences, including genomic instability and cancer. Research on PRIM2A recombinant proteins aims to elucidate its structure, function, and interaction with other replication factors, which can offer insights into the mechanisms of DNA replication and the potential development of therapeutics targeting DNA replication in cancer cells. Furthermore, understanding PRIM2A's interactions and regulatory mechanisms can facilitate advancements in gene editing technologies and improve biotechnological applications. As such, the investigation of PRIM2A not only enhances our fundamental understanding of molecular biology but also holds promise for clinical applications in cancer treatment and genome editing strategies.
