Analytical Data
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基因名
speB
- Application
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别名
speB；GDAH；Guanidino acid hydrolase. mitochondrial
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
B7LFJ6
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表达区间
1-306aa
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氨基酸序列
MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDMATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMSEKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCEFDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDIDNGFTVLDACQVNDRSVDDVIAQVKQIVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVAPAYDQSEITALAAATLALEMLYIQAAKKGE
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分子量
49.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of recombinant SpeB (Streptococcus pyogenes endopeptidase B) is significant due to its role in the pathogenesis of Streptococcus pyogenes, a bacterium responsible for various human diseases, including pharyngitis, scarlet fever, and severe invasive infections like necrotizing fasciitis. SpeB is a cysteine protease that is secreted by the bacteria and plays a crucial role in the breakdown of host tissues, immune evasion, and modulation of inflammation. Understanding the mechanisms of SpeB activity and regulation is essential for developing targeted therapeutic strategies and vaccines. The recombinant production of SpeB allows for detailed structural and functional studies, enabling researchers to investigate its enzymatic properties, substrate specificity, and interactions with host immune components. By utilizing recombinant DNA technology, scientists can produce significant amounts of purified SpeB for biochemical assays and potential applications in diagnostics or therapeutics. Furthermore, studying the potential inhibition of SpeB activity may provide insights into novel antimicrobial strategies against S. pyogenes infections, which remain a significant public health concern globally. This research is crucial for advancing our knowledge of bacterial virulence factors and for exploring avenues to mitigate the impact of Streptococcus pyogenes-related diseases.
