Analytical Data
-
Gene name
PPP4C
- Application
-
Alternative Names
PP X; PP-X; Pp4; PP4C; PP4C_HUMAN; PPH3; PPP4; ppp4c; PPX; protein phosphatase 4 (formerly X). catalytic subunit; Protein phosphatase 4 catalytic subunit; Protein phosphatase X; protein phosphatase X. catalytic subunit; Serine/threonine protein phosphatase 4 catalytic subunit; Serine/threonine-protein phosphatase 4 catalytic subunit
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P60510
-
Expression Region
2-307 aa
-
AA Sequence
AEISDLDRQ IEQLRRCELI KESEVKALCA KAREILVEES NVQRVDSPVT VCGDIHGQFY DLKELFRVGG DVPETNYLFM GDFVDRGFYS VETFLLLLAL KVRYPDRITL IRGNHESRQI TQVYGFYDEC LRKYGSVTVW RYCTEIFDYL SLSAIIDGKI FCVHGGLSPS IQTLDQIRTI DRKQEVPHDG PMCDLLWSDP EDTTGWGVSP RGAGYLFGSD VVAQFNAAND IDMICRAHQL VMEGYKWHFN ETVLTVWSAP NYCYRCGNVA AILELDEHLQ KDFIIFEAAP QETRGIPSKK PVADYFL
-
Molecular Weight
35.0 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PPP4C, or Protein Phosphatase 4 Catalytic Subunit, is a serine/threonine phosphatase that plays a critical role in various cellular processes, including cell division, DNA repair, and response to stress. Research has increasingly highlighted its importance in regulating the cell cycle and maintaining genomic stability, making it a focal point in cancer biology studies. Aberrant PPP4C activity has been implicated in the progression of several cancers, where it may contribute to uncontrolled cell proliferation and resistance to apoptosis. To better understand its functional dynamics, studies have focused on the reconstitution of PPP4C as a recombinant protein. This approach facilitates biochemical assays and structural analyses, providing insights into its enzymatic activity, regulatory mechanisms, and interaction with other cellular proteins. Through effective reconstitution, researchers aim to discern the intricate pathways mediated by PPP4C, paving the way for potential therapeutic interventions targeting its regulatory network in cancer and other diseases characterized by dysregulated cell signaling pathways.
