Analytical Data
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Gene name
mcr1
- Application
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Alternative Names
mcr1；Gpr24；Slc1；Melanin-concentrating hormone receptor 1
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
A0A0R6L508
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Expression Region
1-541aa
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AA Sequence
MMQHTSVWYRRSVSPFVLVASVAVFLTATANLTFFDKISQTYPIADNLGFVLTIAVVLFGAMLLITTLLSSYRYVLKPVLILLLIMGAVTSYFTDTYGTVYDTTMLQNALQTDQAETKDLLNAAFIMRIIGLGVLPSLLVAFVKVDYPTWGKGLMRRLGLIVASLALILLPVVAFSSHYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYHAKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSCGTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLPKAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAYFKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDVSMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDAITPTLLKLFDVTADKVKDRTAFIR
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Molecular Weight
60.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MCR1, or mobile colistin resistance 1, is a plasmid-mediated gene responsible for conferring resistance to colistin, an antibiotic often used as a last-resort treatment for multidrug-resistant bacterial infections. The emergence of MCR1 in various bacterial species, including Escherichia coli and Klebsiella pneumoniae, poses a significant threat to global public health, as it undermines the efficacy of colistin and complicates treatment regimens for severe infections. Research into MCR1 is particularly critical due to its ability to spread rapidly through horizontal gene transfer among bacterial populations. This resistance mechanism not only highlights the urgent need for novel therapeutic approaches but also emphasizes the importance of surveillance and infection control measures in healthcare settings. Understanding the structure and function of MCR1 can aid in the development of strategies to combat colistin resistance, including the design of inhibitors or alternative treatments. Furthermore, studying the evolutionary dynamics and environmental reservoirs of MCR1 can provide insights into its transmission pathways and potential risks to human health. Given the rising incidence of antibiotic resistance, MCR1 research is vital in informing public health policies and promoting responsible antibiotic use to mitigate the threats posed by resistant pathogens.
