Analytical Data
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Gene name
PPP1R16A
- Application
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Alternative Names
2900084E10Rik; Likley ortholog of mouse myosin phosphatase targeting subunit 3; MGC14333; Myosin phosphatase target subunit 3; Myosin phosphatase targeting subunit 3 ; Myosin phosphatase-targeting subunit 3; MYPT3 ; PP16A_HUMAN; PPP1R16A; Protein phosphatase 1 regulatory subunit 16A; Protein phosphatase 1. regulatory (inhibitor) subunit 16A; R75527
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96I34
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Expression Region
1-525 aa
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AA Sequence
MAEHLELLAE MPMVGRMSTQ ERLKHAQKRR AQQVKMWAQA EKEAQGKKGP GERPRKEAAS QGLLKQVLFP PSVVLLEAAA RNDLEEVRQF LGSGVSPDLA NEDGLTALHQ CCIDDFREMV QQLLEAGANI NACDSECWTP LHAAATCGHL HLVELLIASG ANLLAVNTDG NMPYDLCDDE QTLDCLETAM ADRGITQDSI EAARAVPELR MLDDIRSRLQ AGADLHAPLD HGATLLHVAA ANGFSEAAAL LLEHRASLSA KDQDGWEPLH AAAYWGQVPL VELLVAHGAD LNAKSLMDET PLDVCGDEEV RAKLLELKHK HDALLRAQSR QRSLLRRRTS SAGSRGKVVR RVSLTQRTDL YRKQHAQEAI VWQQPPPTSP EPPEDNDDRQ TGAELRPPPP EEDNPEVVRP HNGRVGGSPV RHLYSKRLDR SVSYQLSPLD STTPHTLVHD KAHHTLADLK RQRAAAKLQR PPPEGPESPE TAEPGLPGDT VTPQPDCGFR AGGDPPLLKL TAPAVEAPVE RRPCC
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Molecular Weight
57.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PPP1R16A, also known as Protein Phosphatase 1 Regulatory Subunit 16A, is a pivotal regulatory protein that modulates the activity of Protein Phosphatase 1 (PP1), a key enzyme involved in various cellular processes such as cell division, apoptosis, and metabolism. Alterations in PP1 activity, regulated by its interacting partners like PPP1R16A, have been implicated in numerous diseases, including cancer and neurodegenerative disorders. Understanding the structure and function of PPP1R16A can provide insights into its role in cellular signaling pathways and its potential as a therapeutic target. Research has focused on the recombinant expression of PPP1R16A in various systems to characterize its biochemical properties and elucidate its regulatory mechanisms. Techniques such as X-ray crystallography and NMR spectroscopy are employed to study its interactions with PP1 and other cellular partners. By investigating the functional dynamics of PPP1R16A, researchers aim to uncover novel strategies for disease intervention, highlighting its importance in maintaining cellular homeostasis and the potential for developing specific inhibitors or modulators that can influence PP1 activity through PPP1R16A.
