Analytical Data
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Gene name
DCK
- Application
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Alternative Names
DCK；Deoxycytidine kinase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P27707
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Expression Region
1-260aa
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AA Sequence
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMATPPKRSCPSFSA SSEGTRIKKISIEGNIAAGKSTFVNILKQLCEDWEVVPEPVARWCNVQST QDEFEELTMSQKNGGNVLQMMYEKPERWSFTFQTYACLSRIRAQLASLNG KLKDAEKPVLFFERSVYSDRYIFASNLYESECMNETEWTIYQDWHDWMNN QFGQSLELDGIIYLQATPETCLHRIYLRGRNEEQGIPLEYLEKLHYKHES WLLHRTLKTNFDYLQEVPILTLDVNEDFKDKYESLVEKVKEFLSTL
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Molecular Weight
35 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DCK, or deoxycytidine kinase, is an important enzyme involved in the salvage pathway of nucleotide metabolism, specifically in the phosphorylation of deoxynucleosides into their corresponding monophosphate forms. This enzyme plays a crucial role in the proliferation and survival of cells, particularly in the context of cancer and viral infections, where rapid nucleotide synthesis is vital for the growth of malignant cells or pathogens. Research on the recombination and characterization of DCK has gained prominence due to its potential as a therapeutic target. For instance, DCK's activity is significant in the activation of nucleoside analogs, which are prodrugs used in cancer chemotherapy and antiviral therapy. Variations in DCK expression and activity can influence the efficacy of these treatments, leading to significant research into the enzyme’s genetic and biochemical properties. Additionally, understanding the structure-function relationship of DCK through recombinant protein studies can pave the way for the development of novel inhibitors or enhancers, contributing to more effective therapeutic strategies. Overall, the study of DCK and its recombinant forms holds promise for advancing our knowledge in cancer treatment and antiviral drug development, making it a focal point of biomedical research in recent years.
