Analytical Data
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Gene name
POU3F1
- Application
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Alternative Names
POU domain. class 3. transcription factor 1. Octamer-binding protein 6. Oct-6. Octamer-binding transcription factor 6. OTF-6. POU domain transcription factor SCIP
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q03052
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Expression Region
1-451 aa
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AA Sequence
MATTAQYLPRGPGGGAGGTGPLMHPDAAAAAAAAAAAERLHAGAAYREVQKLMHHEWLGAGAGHPVGLAHPQWLPTGGGGGGDWAGGPHLEHGKAGGGGTGRADDGGGGGGFHARLVHQGAAHAGAAWAQGSTAHHLGPAMSPSPGASGGHQPQPLGLYAQAAYPGGGGGGLAGMLAAGGGGAGPGLHHALHEDGHEAQLEPSPPPHLGAHGHAHGHAHAGGLHAAAAHLHPGAGGGGSSVGEHSDEDAPSSDDLEQFAKQFKQRRIKLGFTQADVGLALGTLYGNVFSQTTICRFEALQLSFKNMCKLKPLLNKWLEETDSSSGSPTNLDKIAAQGRKRKKRTSIEVGVKGALESHFLKCPKPSAHEITGLADSLQLEKEVVRVWFCNRRQKEKRMTPAAGAGHPPMDDVYAPGELGPGGGGASPPSAPPPPPPAALHHHHHHTLPGSVQ
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Molecular Weight
76.01 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POU3F1, also known as Oct-6 or OTF-1, is a member of the POU family of transcription factors that plays crucial roles in various biological processes, including brain development, cellular differentiation, and the maintenance of stem cell characteristics. Its expression is predominantly localized in the central nervous system, where it is essential for neuronal lineage specification and maturation. Recent studies have highlighted POU3F1's involvement in various neural-related diseases, including certain cancers and neurodegenerative disorders, suggesting its potential as a therapeutic target. The recombinant protein of POU3F1 has gained attention in research due to its ability to interact with specific DNA sequences, thereby influencing gene expression patterns. Investigating the functional dynamics of POU3F1 through recombinant technology enables researchers to dissect its mechanistic roles in gene regulation and cellular processes, illuminating its contribution to both normal physiology and pathological conditions. Furthermore, understanding POU3F1’s structure-function relationship could pave the way for novel interventions in diseases linked to neuronal dysfunction. As the field progresses, the exploration of POU3F1 recombinant protein not only advances our knowledge of transcriptional regulation in neuronal biology but also opens avenues for innovative therapeutic approaches in neurobiology and regenerative medicine.
