Analytical Data
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Gene name
POLR3D
- Application
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Alternative Names
POLR3D; BN51; BN51T; DNA-directed RNA polymerase III subunit RPC4; RNA polymerase III subunit C4; DNA-directed RNA polymerase III subunit D; Protein BN51; RNA polymerase III 47 kDa subunit; RPC53 homolog
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P05423
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Expression Region
2-398 aa
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AA Sequence
SEGNAAGEP STPGGPRPLL TGARGLIGRR PAPPLTPGRL PSIRSRDLTL GGVKKKTFTP NIISRKIKEE PKEEVTVKKE KRERDRDRQR EGHGRGRGRP EVIQSHSIFE QGPAEMMKKK GNWDKTVDVS DMGPSHIINI KKEKRETDEE TKQILRMLEK DDFLDDPGLR NDTRNMPVQL PLAHSGWLFK EENDEPDVKP WLAGPKEEDM EVDIPAVKVK EEPRDEEEEA KMKAPPKAAR KTPGLPKDVS VAELLRELSL TKEEELLFLQ LPDTLPGQPP TQDIKPIKTE VQGEDGQVVL IKQEKDREAK LAENACTLAD LTEGQVGKLL IRKSGRVQLL LGKVTLDVTM GTACSFLQEL VSVGLGDSRT GEMTVLGHVK HKLVCSPDFE SLLDHKHR
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Molecular Weight
44.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POLR3D, or Polymerase III D, is a critical enzyme involved in the transcription of various small non-coding RNAs, including tRNA and 5S rRNA, which are essential for protein synthesis and cellular function. Research on POLR3D has gained attention due to its role in transcription regulation and its implications in various diseases, including cancer and genetic disorders. This enzyme is part of the RNA polymerase III complex, which is responsible for synthesizing RNA molecules that are crucial for maintaining cellular homeostasis and functionality. Abnormalities in POLR3D expression or function can disrupt normal gene expression pathways, leading to aberrant cellular behavior. Furthermore, POLR3D has been implicated in the responses to cellular stress and the maintenance of genome integrity. The study of recombinant POLR3D proteins enables researchers to investigate its structural and functional properties, understand its interactions with other cellular components, and elucidate its role in disease mechanisms. This knowledge can pave the way for the development of novel therapeutic strategies targeting POLR3D-related pathways, thereby contributing to advancements in the field of molecular biology and medicine. Overall, the exploration of POLR3D not only enhances our understanding of RNA polymerase functionality but also opens new avenues for innovative treatments for diseases linked to its dysregulation.
