Analytical Data
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Gene name
POLR2G
- Application
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Alternative Names
DNA directed RNA polymerase II 19 kDa polypeptide ; DNA directed RNA polymerase II subunit G; DNA directed RNA polymerase II subunit RPB7; DNA-directed RNA polymerase II subunit G; DNA-directed RNA polymerase II subunit RPB7; hRPB19; hsRPB7; MGC138367; MGC138369; POLR2G; polymerase (RNA) II (DNA directed) polypeptide G; RNA polymerase II 19 kDa subunit; RNA polymerase II subunit B7; RPB19; RPB7 ; RPB7_HUMAN
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P62487
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Expression Region
1-172 aa
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AA Sequence
MFYHISLEHE ILLHPRYFGP NLLNTVKQKL FTEVEGTCTG KYGFVIAVTT IDNIGAGVIQ PGRGFVLYPV KYKAIVFRPF KGEVVDAVVT QVNKVGLFTE IGPMSCFISR HSIPSEMEFD PNSNPPCYKT MDEDIVIQQD DEIRLKIVGT RVDKNDIFAI GSLMDDYLGL VS
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Molecular Weight
19.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
POLR2G, or polymerase II subunit G, is a crucial component of RNA polymerase II (Pol II), the enzyme responsible for synthesizing messenger RNA (mRNA) from DNA templates in eukaryotic cells. Understanding POLR2G is vital as it plays a significant role in gene expression regulation, influencing various cellular processes including cell growth, differentiation, and response to environmental stimuli. Research has shown that alterations in POLR2G expression can affect transcriptional dynamics, which may lead to various diseases, including cancers. The study of POLR2G has become increasingly important in the context of developing therapeutic targets and understanding the molecular mechanisms of transcriptional regulation. Recombinant POLR2G proteins are utilized in laboratory settings to elucidate their functional characteristics, interactions with other transcriptional machinery, and the effects of post-translational modifications. By producing and analyzing these recombinant proteins, researchers aim to decode the complex interactions involved in the transcription process, which may yield insights into the development of novel treatments for transcription-related diseases and enhance our understanding of fundamental biological processes.
