Analytical Data
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Gene name
BUD13
- Application
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Alternative Names
Bud13; BUD13 homolog (S. cerevisiae); BUD13 homolog; BUD13_HUMAN; Cwc26; FLJ27090; fSAP71
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BRD0
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Expression Region
1-458aa
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AA Sequence
MTSKADYLKK YLSPASGDIE KKKKKKNKDK NKPSGLRLIE EDAFLSVDAA KTRDIGSDEE REEIEVLKQS VKKAKVVHGF KQTFAEVDAP KVIKPEPLSP DNSPPRGKRQ RHDSDNSPPR PSRKRNDSDN SPPRPSRNRH DSDKDNSPPR RRRHDSDNSP PRPSRKIREE SPSARNRRSP PRTRRDRHDS DNSPPRNRSR RDSDNSPPRR RPSSPARRRK DDDLSPPRKS RKIEEPKKIK KEEPDSDTET SGRTLEGKRS GLQSARDLKE ESDKLRAKNS KMFEEMDTSV SGRFADTVYR QKQTKKKGKD SEEDQAKKER ETKKTEELKE KYKSWNKGVA QIEDRRAQLE EMARVAAEPM ARARDDDAMN AHLKEVLHAA DPMANMIQKK RRDTAIDRGE LVYPSYHGHF VPNRFGIAPG YRWDGVDRSN GFEGKLAKTE NTKTANQSEY YKSIAEYE
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Molecular Weight
52.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
BUD13 (BUD13 homolog, yeast) is a protein crucial for various cellular processes, particularly in the context of gene expression and RNA metabolism. Originally identified in yeast, BUD13 has gained attention for its role in splicing regulation and the maintenance of mRNA integrity. The protein is part of a larger family of splicing regulators, which are essential for the accurate processing of pre-mRNA into functional mRNA. Misregulation of splicing can lead to various diseases, including cancer and neurodegenerative disorders. In recent years, studies have suggested that BUD13 may have unique functions beyond splicing, potentially influencing cellular stress responses and developmental processes. Understanding the structure and function of recombinant BUD13 protein can provide insights into these complex mechanisms. Additionally, the ability to produce and study BUD13 in a laboratory setting opens doors for therapeutic exploration, particularly in targeting diseases associated with splicing defects. As research advances, the manipulation of BUD13 and its pathways may pave the way for innovative treatments, emphasizing the importance of this protein in cellular health and disease.
