Analytical Data
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Gene name
POLE4
- Application
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Alternative Names
DNA polymerase epsilon 4 accessory subunit; DNA polymerase epsilon p12 subunit; DNA polymerase epsilon subunit 4; DNA polymerase epsilon subunit p12; DNA polymerase II subunit 4; DPOE4_HUMAN; p12; POLE4; Polymerase (DNA directed) epsilon 4 (p12 subunit); Polymerase (DNA directed) epsilon 4; YHHQ1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NR33
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Expression Region
2-117 aa
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AA Sequence
AAAAAAGSG TPREEEGPAG EAAASQPQAP TSVPGARLSR LPLARVKALV KADPDVTLAG QEAIFILARA AELFVETIAK DAYCCAQQGK RKTLQRRDLD NAIEAVDEFA FLEGTLD
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Molecular Weight
12.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POLE4, also known as DNA polymerase epsilon 4, is a crucial component of the DNA replication machinery, particularly involved in the synthesis of the leading strand during DNA replication. Recent studies have highlighted its essential role in maintaining genomic stability and facilitating efficient DNA repair processes. Researchers have focused on the biochemical properties of the POLE4 protein to understand its function in the context of cellular replication stress and oxidative damage. Dysregulation or mutations in POLE4 have been linked to various cancers and genetic disorders, making it a significant target for therapeutic intervention. The structural and functional characterization of the POLE4 protein can provide valuable insights into its mechanism of action and its interactions with other replication factors. Consequently, understanding the role of POLE4 in DNA replication and repair pathways is critical for developing novel strategies to combat cancer and improve the effectiveness of existing treatments. Current research efforts are directed towards elucidating the specific role of POLE4 in different cellular contexts and its potential as a biomarker for tumor progression and response to therapy.
