Analytical Data
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Gene name
PLEKHF1
- Application
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Alternative Names
PLEKHF1；APPD；LAPF；ZFYVE15；Pleckstrin homology domain-containing family F member 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96S99
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Expression Region
1-279aa
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AA Sequence
MVDHLANTEINSQRIAAVESCFGASGQPLALPGRVLLGEGVLTKECRKKAKPRIFFLFNDILVYGSIVLNKRKYRSQHIIPLEEVTLELLPETLQAKNRWMIKTAKKSFVVSAASATERQEWISHIEECVRRQLRATGRPPSTEHAAPWIPDKATDICMRCTQTRFSALTRRHHCRKCGFVVCAECSRQRFLLPRLSPKPVRVCSLCYRELAAQQRQEEAEEQGAGSPGQPAHLARPICGASSGDDDDSDEDKEGSRDGDWPSSVEFYASGVAWSAFHS
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Molecular Weight
35.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PLEKHF1 (Pleckstrin Homology Domain Containing Family F Member 1) is a relatively understudied member of the pleckstrin homology (PH) domain-containing protein family, which is known for its involvement in diverse cellular processes, including signal transduction, cell migration, and membrane trafficking. The significance of PLEKHF1 lies in its potential roles in both physiological and pathophysiological contexts, particularly in the immune response, cancer biology, and neurodevelopment. Recent studies have started to uncover its expression patterns in various tissues and cell types, indicating its potential as a biomarker in certain diseases. The development of recombinant PLEKHF1 protein allows researchers to investigate its structure-function relationships and biochemical properties, providing insights into its mechanism of action. This research is crucial for understanding how PLEKHF1 may contribute to cell signaling pathways and potentially serve as a therapeutic target. By employing techniques such as X-ray crystallography and biophysical assays, scientists are beginning to elucidate the functional roles of PLEKHF1 at the molecular level, paving the way for future studies to explore its implications in health and disease. As interest in PLEKHF1 continues to grow, further investigation could reveal novel aspects of cell biology, offering opportunities for innovative therapeutic strategies.
