Analytical Data
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Gene name
POLDIP3
- Application
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Alternative Names
46 kDa DNA polymerase delta interaction protein; DNA polymerase delta interacting protein 3; KIAA1649; P46; PDIP3_HUMAN; PDIP46; POLDIP3; Polymerase (DNA-directed). delta interacting protein 3; Polymerase delta interacting protein 46; Polymerase delta-interacting protein 3; RNA-binding protein P46 ; S6K1 Aly/REF-like target; SKAR
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BY77
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Expression Region
2-421 aa
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AA Sequence
ADISLDELI RKRGAAAKGR LNARPGVGGV RSRVGIQQGL LSQSTRTATF QQRFDARQKI GLSDARLKLG VKDAREKLLQ KDARFRIKGK VQDAREMLNS RKQQTTVPQK PRQVADAREK ISLKRSSPAA FINPPIGTVT PALKLTKTIQ VPQQKAMAPL HPHPAGMRIN VVNNHQAKQN LYDLDEDDDG IASVPTKQMK FAASGGFLHH MAGLSSSKLS MSKALPLTKV VQNDAYTAPA LPSSIRTKAL TNMSRTLVNK EEPPKELPAA EPVLSPLEGT KMTVNNLHPR VTEEDIVELF CVCGALKRAR LVHPGVAEVV FVKKDDAITA YKKYNNRCLD GQPMKCNLHM NGNVITSDQP ILLRLSDSPS MKKESELPRR VNSASSSNPP AEVDPDTILK ALFKSSGASV TTQPTEFKIK L
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Molecular Weight
46.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POLDIP3, or PCNA-Dependent Protein 3, is an important protein involved in cellular processes such as DNA replication, repair, and transcription regulation. It has garnered attention due to its role in maintaining genomic stability and its potential implications in cancer development. POLDIP3 has been shown to interact with proliferating cell nuclear antigen (PCNA), a key protein that acts as a sliding clamp during DNA synthesis, thus facilitating the coordination of DNA polymerases and other factors necessary for effective replication and repair. Research on POLDIP3 has increased as scientists aim to elucidate its functional mechanisms and regulatory pathways. Understanding the biochemical properties and interactions of POLDIP3 through recombinant protein techniques offers insights into its role in cell cycle control and DNA damage response. The recombinant expression of POLDIP3 can enable advanced studies using various biophysical methods, such as crystallography and NMR spectroscopy, to determine its structural characteristics and interactome. Moreover, investigating POLDIP3 in disease contexts, particularly in tumorigenesis, underscores its potential as a biomarker and therapeutic target. As our comprehension of POLDIP3 continues to expand, it may provide novel strategies for cancer treatment and the development of innovative therapeutic interventions aimed at enhancing DNA repair mechanisms or inhibiting aberrant cell proliferation.
