Analytical Data
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Gene name
PMS2L5
- Application
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Alternative Names
PMS2P5; PMS2L5; PMS4; PMS7; Postmeiotic segregation increased 2-like protein 5; Postmeiotic segregation increased protein 4; Postmeiotic segregation increased protein 7; Putative postmeiotic segregation increased 2 pseudogene 5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
A8MQ11
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Expression Region
1-134 aa
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AA Sequence
MWGRRRKLRR LNDVTISTCH VSAKVGTRLV FDHDGKIIQK TPYPHPRGTT VSVKQLFSTL PVRHKEFQRN IKKKRACFPF AFCRDCQFLE GSPAMLPVQP AKLTPRSTPP HPCSLEDNVI TVFSSVKNGP GSSR
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Molecular Weight
15.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PMS2L5 is a member of the PMS (Postmeiotic Segregation Increased 2) gene family, which plays a crucial role in DNA repair and maintaining genomic stability. The significance of this gene has been underscored by its involvement in the mismatch repair (MMR) pathway, which is essential for repairing DNA replication errors that could lead to mutations and genomic instability. Research has shown that defects in MMR genes like PMS2L5 can lead to a higher predisposition to various cancers, particularly colorectal cancer, highlighting the importance of understanding its functional mechanisms. Given the emerging role of PMS2L5 in both hereditary and sporadic forms of cancer, ongoing studies aim to elucidate its structure and catalytic properties through recombinant protein expression and purification methods. By generating and characterizing recombinant PMS2L5 protein, researchers can better understand its biochemical activities, interaction partners, and overall contribution to cellular processes involved in DNA repair. This knowledge may provide insights into the development of targeted therapies for cancers associated with MMR deficiencies. Overall, the investigation of PMS2L5 as a recombinant protein serves as a vital step in unraveling its biological roles and therapeutic potential in cancer research.
