Analytical Data
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Gene name
PJA1
- Application
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Alternative Names
E3 ubiquitin-protein ligase Praja 1; E3 ubiquitin-protein ligase Praja-1; PJA1; PJA1_HUMAN; PRAJA 1; Praja ring finger 1; Praja1; RING finger protein 70; RNF 70; RP23-414J22.4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8NG27
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Expression Region
1-643 aa
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AA Sequence
MGQESSKPVW PNPTGGYQSN TGRRYGRRHA YVSFRPPTSQ RERIASQRKT NSEVPMHRSA PSQTTKRSRS PFSTTRRSWD DSESSGTNLN IDNEDYSRYP PREYRASGSR RGMAYGHIDS YGADDSEEEG AGPVERPPVR GKTGKFKDDK LYDPEKGARS LAGPPPHFSS FSRDVREERD KLDPVPAARC SASRADFLPQ SSVASQSSSE GKLATKGDSS ERERREQNLP ARPSRAPVSI CGGGENTSKS AEEPVVRPKI RNLASPNCVK PKIFFDTDDD DDMPHSTSRW RDTANDNEGH SDGLARRGRG ESSSGYPEPK YPEDKREARS DQVKPEKVPR RRRTMADPDF WTHSDDYYKY CDEDSDSDKE WIAALRRKYR SREQTLSSSG ESWETLPGKE EREPPQAKVS ASTGTSPGPG ASASAGAGAG ASAGSNGSNY LEEVREPSLQ EEQASLEEGE IPWLQYHEND SSSEGDNDSG HELMQPGVFM LDGNNNLEDD SSVSEDLEVD WSLFDGFADG LGVAEAISYV DPQFLTYMAL EERLAQAMET ALAHLESLAV DVEVANPPAS KESIDALPEI LVTEDHGAVG QEMCCPICCS EYVKGEVATE LPCHHYFHKP CVSIWLQKSG TCPVCRCMFP PPL
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Molecular Weight
71.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PJA1, or PJA1 protein, is a member of the RING-finger protein family and plays a critical role in cellular processes such as protein degradation, signaling pathways, and the maintenance of protein homeostasis. Its function has garnered attention due to its involvement in the ubiquitin-proteasome system, where it acts as a crucial E3 ubiquitin ligase, facilitating the tagging of substrate proteins for degradation. The study of PJA1 is particularly significant in the context of various diseases, including cancer, where dysregulation of protein degradation pathways can contribute to tumorigenesis. Additionally, PJA1 has been linked to neurodegenerative conditions, reinforcing its importance in maintaining cellular health and stability. Research into the recombinant expression of PJA1 offers insights into its structural and functional properties, enabling the exploration of its role in disease mechanisms. By generating high-purity recombinant PJA1, scientists can investigate its interactions with substrates and other regulatory proteins, shedding light on its potential as a therapeutic target. Understanding the intricacies of PJA1's functions and its implications in disease pathology remains a pivotal area of research, with potential applications in drug development and targeted therapy strategies. As such, the ongoing study of PJA1 not only deepens our understanding of fundamental biological processes but also opens avenues for novel interventions in disease management.
