Analytical Data
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Gene name
GSPT1
- Application
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Alternative Names
GSPT1；ERF3A；Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P15170
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Expression Region
1-499aa
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AA Sequence
MELSEPIVENGETEMSPEESWEHKEEISEAEPGGGSLGDGRPPEESAHEMMEEEEEIPKPKSVVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDTVAPGENLKIRLKGIEEEEILPGFILCDPNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD
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Molecular Weight
59.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GSPT1, or G1 to S phase transition 1, is a crucial protein involved in the regulation of cell cycle progression, particularly in the transition from the G1 phase to the S phase. Its role as an essential factor in translation termination and mRNA surveillance makes it significant in cellular processes, particularly in response to stress signals and during cellular differentiation. Research into GSPT1 has gained momentum due to its implications in various diseases, including cancer, where its dysregulation can lead to uncontrolled cell proliferation. Furthermore, GSPT1 has been associated with innate immune responses, making it a point of interest in studies related to infectious diseases. The recombinant expression of GSPT1 allows for detailed investigations into its structural and functional properties, facilitating the understanding of its mechanisms in cellular processes and disease states. This research could pave the way for developing therapeutic strategies targeting GSPT1, especially in cancers where this protein is implicated. The study of GSPT1 not only elucidates its biological significance but also enhances the understanding of cellular responses to environmental changes, thus providing a potential avenue for novel interventions in disease treatment and management.
