Analytical Data
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Gene name
PIPSL
- Application
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Alternative Names
PIPSL; PSMD4P2; Putative PIP5K1A and PSMD4-like protein; PIP5K1A-PSMD4
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
A2A3N6
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Expression Region
1-862 aa
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AA Sequence
MASEVPYASGMPIKKIGHRSVDSSGGTTSSALKGAIQLGITHTVGSLSTKPESDVLMQDFHMVESIFFPSEGSNLTPAHHYNAFRFKTYAPVAFRYFWELFGIRPDDYLYSLCSEPLIELCSSGASGSLFYVSSDDEFIVKTVRHKEAEFLQKLLPGYYINLNQNPRTLLPKFYGLYCVQTGGKNIRIVVMNNLLPRSVKMHIKYDLKGSTYRRRASQKEREKPLPTFKDLDFLQDIPDGLFLDADVHNALCKTLQRDCLVLQSFKIMDYSLLMSIHNIDHAQREPLSSETQYSVDTRRPAPQKALYSTAMESIQGEARRGGTMETDDHMGGIPARNSKGERLLLYIGIIDILQSYRFVKKLEHSWKALIHDGDTVSVHRPGFYAEWFQRFMCNTVFKKIPLKPSPSKKLRSGSSFSQRAGSSGNSCITYKPLVSGEHKAQVTTKAEVEPGVHLGCPDVLPQTPPLEEISEGSPTPDPSFSPLVEETLQMLTTSVDNSEYMGNGDFLPTRLQAQQDAVNTVCHSKTRSNPENNVGLITLDNDCEVLTTLTPDTGRILSKLHTVQPKGKITFCMGIHVAHLALKHRQGNNHKIRIIAFVGNPVEDNEKNLVKLAKCLKKEKVNVDIINFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISFPILAGEGGAMMGLGASDFEFGVDPSADPELALVLRVFMEEQRQRQEEEARQAAAASAAEAGIATTGTEDSDDALLKMTISQQEFGHTGLPDLSSMTEEEKIVCAMQMSLQGAEFGLAESADIDASSAMDTSEPAKEEDDYDVMQDPEFLQSVLENLPGVDPNNEAIRNAVGSLASQATKDSKKDKKEEDEK
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Molecular Weight
121.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of PIPSL (Phosphatidylinositol Phosphate-Specific Lipase) recombinant proteins has garnered significant attention due to their crucial roles in cellular signaling and membrane dynamics. PIPSL enzymes are involved in the hydrolysis of phosphatidylinositol phosphates, a group of lipids that serve as key signaling molecules in various cellular processes, including cell growth, apoptosis, and cytoskeletal rearrangements. The disruption of PIPSL activity has been implicated in several diseases, including cancer and neurodegenerative disorders, making them crucial targets for therapeutic development. The ability to produce recombinant PIPSL proteins has enabled researchers to investigate their structure-function relationships, enzymatic mechanisms, and interactions with other cellular components. Through the use of advanced techniques, such as X-ray crystallography and cryo-electron microscopy, scientists aim to elucidate how these proteins contribute to cellular signaling pathways and how their dysregulation may lead to pathological conditions. Moreover, engineered PIPSL proteins are being explored for potential applications in targeted drug delivery and synthetic biology, underscoring the importance of understanding their properties. Overall, the research on PIPSL recombinant proteins is not only pivotal for basic biological understanding but also holds promise for innovative medical solutions in treating diseases linked to lipid signaling pathways.
