Analytical Data
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Gene name
PIH1D2
- Application
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Alternative Names
PIH1D2; PIH1 domain-containing protein 2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8WWB5
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Expression Region
1-315 aa
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AA Sequence
METSSKGLLT QVTQFWNLLD DLAQSDPEGY EKFIQQQLKE GKQLCAAPEP QLCLQTRILK PKEKILFINL CQWTRIPAPQ STTHPVPLTV GKPEDTTEIS DAYTVIDVAY NPDVLHAAEK DQVKKNQLIQ MAMKCIEEKF QFTLSHSYHI TKFRIKGSIQ RMKQNLMGIQ TDSIDLREKM RRELTLGQIR SSTMSNPDHF PQLLLPKDQV SGKAVCLIEE ISSTEIQVEM KMPAYELKIV HDHSEKPLKI ELKVELPGIN SVSLCDLSVS EDDLLIEVSE KYRLHLNLPK LIDTEMTTAK FIKEKSTLII TMPLV
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Molecular Weight
35.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The PIH1D2 protein is of significant interest in the field of molecular biology and biochemistry due to its potential role in various cellular processes, including protein homeostasis and the regulation of the proteasome, a key component in the degradation of ubiquitinated proteins. Research has shown that PIH1D2 is involved in the assembly and function of the proteasome lid subcomplex, thereby impacting the pathway of protein degradation, which is crucial for maintaining cellular protein levels and quality control. Dysregulation of this process can contribute to various diseases, including cancer and neurodegenerative disorders. Additionally, PIH1D2 has been implicated in several signaling pathways and cellular responses, making it a potential target for therapeutic intervention. Understanding the structure and function of the PIH1D2 protein through recombinant protein studies is essential for unraveling its mechanistic roles in these pathways. The exploration of PIH1D2's interactions with other proteins and its contributions to larger protein complexes may provide valuable insights into its biological significance and potential implications in health and disease, paving the way for novel treatment strategies that harness its functions. Thus, the study of PIH1D2 not only enhances our comprehension of fundamental cellular processes but also offers prospects for innovative therapeutic developments.











