Analytical Data
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基因名
RPL27
- Application
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别名
RPL27;Large ribosomal subunit Protein eL27
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P61353
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表达区间
1-136aa
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氨基酸序列
MGKFMKPGKV VLVLAGRYSG RKAVIVKNID DGTSDRPYSH ALVAGIDRYP RKVTAAMGKK KIAKRSKIKS FVKVYNYNHL MPTRYSVDIP LDKTVVNKDV FRDPALKRKA RREAKVKFEE RYKTGKNKWF FQKLRF
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分子量
15.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RPL27, a ribosomal protein belonging to the large subunit of the ribosome, plays a critical role in the protein synthesis machinery of eukaryotic cells. Its importance extends beyond structure, as it is involved in various cellular processes, including cell growth, proliferation, and stress responses. Recent studies have highlighted the potential link between RPL27 expression levels and various diseases, particularly certain cancers, where its overexpression has been correlated with aggressive tumor behavior and poor prognosis. This has spurred interest in RPL27 as a potential biomarker for cancer diagnostics and a target for therapeutic interventions. Researchers have been focusing on the recombinant expression of RPL27 to facilitate a deeper understanding of its functional roles and interactions within the ribosome and with other cellular components. Utilizing recombinant DNA technology allows for the production of pure RPL27 protein, which can be used in various assays to study its biochemical properties and its involvement in translational regulation. Moreover, investigating the structural characteristics of RPL27 through techniques such as X-ray crystallography or cryo-electron microscopy can provide insights into its contribution to ribosome assembly and function. The ongoing research on RPL27 is significant, as it not only enhances our comprehension of ribosomal biogenesis but also sheds light on its implications in human health, paving the way for the development of novel therapeutic strategies that target ribosomal dysfunction in cancer and other diseases.












