Analytical Data
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基因名
PIGZ
- Application
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别名
PIGZ; SMP3; GPI mannosyltransferase 4; EC 2.4.1.-; GPI mannosyltransferase IV; GPI-MT-IV; Phosphatidylinositol-glycan biosynthesis class Z protein; PIG-Z; SMP3 homolog; hSMP3
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q86VD9
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表达区间
1-579 aa
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氨基酸序列
MQICGSSVASVAAGTSFQVLGPVCWQQLDLKMAVRVLWGGLSLLRVLWCLLPQTGYVHPDEFFQSPEVMAEDILGVQAARPWEFYPSSSCRSVLFPLLISGSTFWLLRLWEELGPWPGLVSGYALLVGPRLLLTALSFALDGAVYHLAPPMGADRWNALALLSGSYVTLVFYTRTFSNTIEGLLFTWLLVLVSSHVTWGPTRKEPAPGPRWRSWLLGGIVAAGFFNRPTFLAFAVVPLYLWGTRGATNPGLKSLTREALVLLPGATLTAAVFVATDSWYFSSPATSRNLVLTPVNFLHYNLNPQNLARHGTHARLTHLAVNGFLLFGVLHAQALQAAWQQLQVGLQASAQMGLLRALGARSLLSSPRSYLLLLYFMPLALLSAFSHQEARFLIPLLVPLVLLCSPQTQPVPWKGTVVLFNALGALLFGCLHQGGLVPGLEYLEQVVHAPVLPSTPTHYTLLFTHTYMPPRHLLHLPGLGAPVEVVDIGGTEDWALCQTLKSFTRQPACQVAGGPWLCRLFVVTPGTTRRAVEKCSFPFKNETLLFPHLTLEDPPALSSLLSGAWRDHLSLHIVELGEET
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分子量
89.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PIGZ (Phosphatidylinositol glycan anchor biosynthesis class Z) is a critical enzyme involved in the post-translational modification of proteins, specifically in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors, which play an essential role in anchoring proteins to cell membranes. Research has highlighted the significance of PIGZ in various biological processes, including cell signaling, adhesion, and immune responses. Mutations or dysregulation of PIGZ can lead to various diseases, including certain types of cancer and developmental disorders. The study of PIGZ-recombinant proteins has gained momentum as it provides insights into the enzyme's structure-function relationship and its interactions with other cellular components. By employing advanced techniques such as recombinant DNA technology, researchers can produce PIGZ proteins for biochemical characterization, high-throughput screening, and potential therapeutic applications. Understanding the functional mechanisms of PIGZ not only enhances our knowledge of GPI-anchor biosynthesis but also paves the way for novel therapeutic strategies targeting GPI-anchored proteins in human diseases. Overall, PIGZ represents a promising area of study within the broader field of cell biology and molecular medicine, emphasizing the intricate connections between protein modifications and cellular functionality.
