Analytical Data
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Gene name
ZWILCH
- Application
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Alternative Names
ZWILCH；Protein zwilch homolog
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H900
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Expression Region
1-591aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMGSMWERLNCAAEDFYSRLLQKFNEEKKGI RKDPFLYEADVQVQLISKGQPNPLKNILNENDIVFIVEKVPLEKEETSHI EELQSEETAISDFSTGENVGPLALPVGKARQLIGLYTMAHNPNMTHLKIN LPVTALPPLWVRCDSSDPEGTCWLGAELITTNNSITGIVLYVVSCKADKN YSVNLENLKNLHKKRHHLSTVTSKGFAQYELFKSSALDDTITASQTAIAL DISWSPVDEILQIPPLSSTATLNIKVESGEPRGPLNHLYRELKFLLVLAD GLRTGVTEWLEPLEAKSAVELVQEFLNDLNKLDGFGDSTKKDTEVETLKH DTAAVDRSVKRLFKVRSDLDFAEQLWCKMSSSVISYQDLVKCFTLIIQSL QRGDIQPWLHSGSNSLLSKLIHQSYHGTMDTVSLSGTIPVQMLLEIGLDK LKKDYISFFIGQELASLNHLEYFIAPSVDIQEQVYRVQKLHHILEILVSC MPFIKSQHELLFSLTQICIKYYKQNPLDEQHIFQLPVRPTAVKNLYQSEK PQKWRVEIYSGQKKIKTVWQLSDSSPIDHLNFHKPDFSELTLNGSLEERI FFTNMVTCSQVHFK
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Molecular Weight
70 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ZWILCH is a protein that plays a crucial role in the regulation of cell division, particularly in the proper functioning of the spindle assembly checkpoint during mitosis. Research on ZWILCH has gained prominence due to its involvement in ensuring accurate chromosome segregation, which is vital for maintaining genomic stability. Aberrations in this process can lead to aneuploidy and are often implicated in various cancers. Studies have highlighted ZWILCH's interactions with other key proteins involved in the mitotic apparatus, shedding light on its function and importance in cellular processes. The understanding of ZWILCH's role has implications not only for basic cell biology but also for potential therapeutic approaches in cancer treatment, where targeting the mechanisms of cell division could enhance the efficacy of existing therapies. As such, investigations into the structure, function, and regulation of ZWILCH continue to be an active area of research, aiming to unravel the complexities of mitotic regulation and its implications for disease.
