Analytical Data
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Gene name
Visfatin
- Application
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Alternative Names
Visfatin；PBEF；Nicotinamide phosphoribosyltransferase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P43490
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Expression Region
2-491aa
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AA Sequence
GPLGSNPAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTEN SKLRKVKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKDVYKEHFQDDVF NEKGWNYILEKYDGHLPIEIKAVPEGFVIPRGNVLFTVENTDPECYWLTN WIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYR GVSSQETAGIGASAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAE HSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLR HLIVSRSTQAPLIIRPDSGNPLDTVLKVLEILGKKFPVTENSKGYKLLPP YLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLN CSFKCSYVVTNGLGINVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLEEG KGDLEEYGQDLLHTVFKNGKVTKSYSFDEIRKNAQLNIELEAAHH
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Molecular Weight
56 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Visfatin, also known as nicotinamide adenine dinucleotide (NAD)-dependent enzyme, has garnered significant interest in recent years due to its multifaceted roles in metabolism, inflammation, and cellular signaling. Initially identified as an adipokine secreted by visceral fat, Visfatin is implicated in various physiological processes, including glucose homeostasis and insulin sensitivity. Its structural similarity to the enzyme pre-B-cell colony-enhancing factor (PBEF) has raised questions about its enzymatic functions and potential as a therapeutic target in metabolic disorders such as obesity and type 2 diabetes. Research has indicated a correlation between elevated levels of Visfatin and obesity-related complications, suggesting its potential as a biomarker for metabolic syndrome. The recombinant protein form of Visfatin has been developed for various experimental applications, allowing researchers to elucidate its mechanisms of action, explore its roles in cell signaling pathways, and assess its impact on energy metabolism. Additionally, studies involving Visfatin recombinant protein have provided insights into its interactions with insulin and other adipokines, furthering our understanding of adipose tissue function and systemic metabolic regulation. As such, the study of Visfatin recombinant protein not only enhances our knowledge of endocrine regulation but also opens avenues for novel therapeutic strategies to combat metabolic diseases.
