Analytical Data
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基因名
Visfatin
- Application
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别名
Visfatin；PBEF；Nicotinamide phosphoribosyltransferase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P43490
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表达区间
2-491aa
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氨基酸序列
GPLGSNPAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTEN SKLRKVKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKDVYKEHFQDDVF NEKGWNYILEKYDGHLPIEIKAVPEGFVIPRGNVLFTVENTDPECYWLTN WIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYR GVSSQETAGIGASAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAE HSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLR HLIVSRSTQAPLIIRPDSGNPLDTVLKVLEILGKKFPVTENSKGYKLLPP YLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLN CSFKCSYVVTNGLGINVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLEEG KGDLEEYGQDLLHTVFKNGKVTKSYSFDEIRKNAQLNIELEAAHH
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分子量
56 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Visfatin, also known as nicotinamide adenine dinucleotide (NAD)-dependent enzyme, has garnered significant interest in recent years due to its multifaceted roles in metabolism, inflammation, and cellular signaling. Initially identified as an adipokine secreted by visceral fat, Visfatin is implicated in various physiological processes, including glucose homeostasis and insulin sensitivity. Its structural similarity to the enzyme pre-B-cell colony-enhancing factor (PBEF) has raised questions about its enzymatic functions and potential as a therapeutic target in metabolic disorders such as obesity and type 2 diabetes. Research has indicated a correlation between elevated levels of Visfatin and obesity-related complications, suggesting its potential as a biomarker for metabolic syndrome. The recombinant protein form of Visfatin has been developed for various experimental applications, allowing researchers to elucidate its mechanisms of action, explore its roles in cell signaling pathways, and assess its impact on energy metabolism. Additionally, studies involving Visfatin recombinant protein have provided insights into its interactions with insulin and other adipokines, furthering our understanding of adipose tissue function and systemic metabolic regulation. As such, the study of Visfatin recombinant protein not only enhances our knowledge of endocrine regulation but also opens avenues for novel therapeutic strategies to combat metabolic diseases.
