Analytical Data
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Gene name
PAX4
- Application
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Alternative Names
KPD; MGC129960; MODY9; Paired box 4; Paired box gene 4; paired box homeotic gene 4; Paired box protein Pax-4; Paired domain gene 4; Pax4; PAX4_HUMAN
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O43316
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Expression Region
1-350 aa
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AA Sequence
MHQDGISSMN QLGGLFVNGR PLPLDTRQQI VRLAVSGMRP CDISRILKVS NGCVSKILGR YYRTGVLEPK GIGGSKPRLA TPPVVARIAQ LKGECPALFA WEIQRQLCAE GLCTQDKTPS VSSINRVLRA LQEDQGLPCT RLRSPAVLAP AVLTPHSGSE TPRGTHPGTG HRNRTIFSPS QAEALEKEFQ RGQYPDSVAR GKLATATSLP EDTVRVWFSN RRAKWRRQEK LKWEMQLPGA SQGLTVPRVA PGIISAQQSP GSVPTAALPA LEPLGPSCYQ LCWATAPERC LSDTPPKACL KPCWDCGSFL LPVIAPSCVD VAWPCLDASL AHHLIGGAGK ATPTHFSHWP
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Molecular Weight
37.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PAX4 is a member of the paired box (PAX) family of transcription factors, which play a crucial role in the development and function of various tissues, particularly in the endocrine system. Research into PAX4 has gained prominence due to its significant involvement in the differentiation of pancreatic beta cells, which are essential for insulin secretion and the regulation of glucose metabolism. Mutations in the PAX4 gene have been linked to several forms of diabetes, including Maturity-Onset Diabetes of the Young (MODY) and Type 2 Diabetes, highlighting its potential as a therapeutic target. PAX4’s expression is finely tuned during embryogenesis and in adult tissues, regulated by various signaling pathways. The recombinant PAX4 protein is often studied to elucidate its molecular mechanisms and interactions within cellular contexts, providing insights into its role in beta-cell function and survival. Furthermore, the production of PAX4 as a recombinant protein allows for the exploration of its potential applications in regenerative medicine and gene therapy, particularly in the context of pancreatic islet cell regeneration and diabetes treatment strategies. Understanding PAX4’s structure and function at a molecular level is crucial for developing novel therapeutic approaches aimed at modulating its activity to enhance insulin production or protect against beta-cell apoptosis, which could ultimately contribute to new interventions for diabetes management. As research progresses, the therapeutic implications of PAX4 continue to attract attention, positioning it as a valuable focus in the landscape of diabetes research and treatment innovation.
