Analytical Data
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基因名
TAS1R1
- Application
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别名
TAS1R1；GPR70；T1R1；TR1；Taste receptor type 1 member 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q7RTX1
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表达区间
21-567aa
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氨基酸序列
FACHSTESSPDFTLPGDYLLAGLFPLHSGCLQVRHRPEVTLCDRSCSFNEHGYHLFQAMRLGVEEINNSTALLPNITLGYQLYDVCSDSANVYATLRVLSLPGQHHIELQGDLLHYSPTVLAVIGPDSTNRAATTAALLSPFLVPMISYAASSETLSVKRQYPSFLRTIPNDKYQVETMVLLLQKFGWTWISLVGSSDDYGQLGVQALENQATGQGICIAFKDIMPFSAQVGDERMQCLMRHLAQAGATVVVVFSSRQLARVFFESVVLTNLTGKVWVASEAWALSRHITGVPGIQRIGMVLGVAIQKRAVPGLKAFEEAYARADKKAPRPCHKGSWCSSNQLCRECQAFMAHTMPKLKAFSMSSAYNAYRAVYAVAHGLHQLLGCASGACSRGRVYPWQLLEQIHKVHFLLHKDTVAFNDNRDPLSSYNIIAWDWNGPKWTFTVLGSSTWSPVQLNINETKIQWHGKDNQVPKSVCSSDCLEGHQRVVTGFHHCCFECVPCGAGTFLNKSDLYRCQPCGKEEWAPEGSQTCFPRTVVFLALREHTS
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分子量
68.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The TAS1R1 gene encodes a critical component of the sweet taste receptor, which is part of the larger TAS1R family responsible for taste perception. The TAS1R1 protein forms a heterodimer with TAS1R3 to facilitate the recognition and transduction of sweet and umami tastes, playing a significant role in dietary preferences and nutrition. Understanding the function of TAS1R1 and its interactions within the taste signaling pathways is vital, as it influences food intake and can be linked to metabolic disorders and obesity. Research involving recombinant TAS1R1 protein is crucial for elucidating its structural and functional properties, as well as its associated signaling mechanisms. By expressing and purifying this protein, scientists can conduct assays to investigate its binding affinities with various ligands, including amino acids and sweeteners, and to explore its role in cellular signaling processes. This research has implications for developing better strategies in food science, nutrition, and public health, particularly concerning managing taste-related issues and improving dietary practices. Additionally, studies on TAS1R1 provide insights into evolutionary adaptations in taste perception across different species, highlighting the broader implications of taste receptor research in understanding organismal behavior and ecology. Overall, investigating TAS1R1 recombinant protein serves as a fundamental step in unveiling the complexities of taste biology and its impact on human health.
