Analytical Data
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Gene name
PAPSS2
- Application
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Alternative Names
PAPSS2; ATPSK2Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2; PAPS synthase 2; PAPSS 2; Sulfurylase kinase 2; SK 2; SK2) [Includes: Sulfate adenylyltransferase; EC 2.7.7.4; ATP-sulfurylase; Sulfate adenylate transferase; SAT); Adenylyl-sulfate kinase; EC 2.7.1.25; 3'-phosphoadenosine-5'-phosphosulfate synthase; APS kinase; Adenosine-5'-phosphosulfate 3'-phosphotransferase; Adenylylsulfate 3'-phosphotransferase)]
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O95340
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Expression Region
1-614 aa
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AA Sequence
MSGIKKQKTE NQQKSTNVVY QAHHVSRNKR GQVVGTRGGF RGCTVWLTGL SGAGKTTISF ALEEYLVSHA IPCYSLDGDN VRHGLNRNLG FSPGDREENI RRIAEVAKLF ADAGLVCITS FISPFAKDRE NARKIHESAG LPFFEIFVDA PLNICESRDV KGLYKRARAG EIKGFTGIDS DYEKPETPER VLKTNLSTVS DCVHQVVELL QEQNIVPYTI IKDIHELFVP ENKLDHVRAE AETLPSLSIT KLDLQWVQVL SEGWATPLKG FMREKEYLQV MHFDTLLDDG VINMSIPIVL PVSAEDKTRL EGCSKFVLAH GGRRVAILRD AEFYEHRKEE RCSRVWGTTC TKHPHIKMVM ESGDWLVGGD LQVLEKIRWN DGLDQYRLTP LELKQKCKEM NADAVFAFQL RNPVHNGHAL LMQDTRRRLL ERGYKHPVLL LHPLGGWTKD DDVPLDWRMK QHAAVLEEGV LDPKSTIVAI FPSPMLYAGP TEVQWHCRSR MIAGANFYIV GRDPAGMPHP ETKKDLYEPT HGGKVLSMAP GLTSVEIIPF RVAAYNKAKK AMDFYDPARH NEFDFISGTR MRKLAREGEN PPDGFMAPKA WKVLTDYYRS LEKN
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Molecular Weight
69.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PAPSS2 (3'-phosphoadenosine-5'-phosphosulfate synthase 2) is a vital enzyme involved in sulfate metabolism, playing a key role in the biosynthesis of sulfated carbohydrates and other sulfated molecules, which are crucial for various biological processes. Mutations in the PAPSS2 gene are linked to skeletal abnormalities, such as the rare condition known as "sulfate transport deficiency," underscoring its significance in human health. Recent studies have focused on the recombinant expression of PAPSS2 to better understand its enzymatic mechanisms and regulatory pathways. The ability to produce PAPSS2 in vitro allows researchers to investigate its structural and functional characteristics, providing insights into its role in sulfation processes. Understanding PAPSS2 at the molecular level could open avenues for therapeutic interventions aimed at alleviating the effects of associated genetic disorders. Moreover, recombinant PAPSS2 can serve as a valuable tool in glycomics research, enhancing our understanding of sulfate modifications on glycoproteins and proteoglycans, which have implications for cell signaling and development. The exploration of PAPSS2's function and regulation through recombinant protein studies is expected to contribute significantly to the broader field of glycobiology and its applications in medicine.
