Analytical Data
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基因名
PAPOLA
- Application
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别名
MGC5378 ; PAP alpha; PAP; PAP-alpha; PapIII; PAPOA_HUMAN; PAPOLA; Plap; Poly(A) polymerase alpha; Polynucleotide adenylyltransferase alpha
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P51003
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表达区间
1-745 aa
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氨基酸序列
MPFPVTTQGS QQTQPPQKHY GITSPISLAA PKETDCVLTQ KLIETLKPFG VFEEEEELQR RILILGKLNN LVKEWIREIS ESKNLPQSVI ENVGGKIFTF GSYRLGVHTK GADIDALCVA PRHVDRSDFF TSFYDKLKLQ EEVKDLRAVE EAFVPVIKLC FDGIEIDILF ARLALQTIPE DLDLRDDSLL KNLDIRCIRS LNGCRVTDEI LHLVPNIDNF RLTLRAIKLW AKRHNIYSNI LGFLGGVSWA MLVARTCQLY PNAIASTLVH KFFLVFSKWE WPNPVLLKQP EECNLNLPVW DPRVNPSDRY HLMPIITPAY PQQNSTYNVS VSTRMVMVEE FKQGLAITDE ILLSKAEWSK LFEAPNFFQK YKHYIVLLAS APTEKQRLEW VGLVESKIRI LVGSLEKNEF ITLAHVNPQS FPAPKENPDK EEFRTMWVIG LVFKKTENSE NLSVDLTYDI QSFTDTVYRQ AINSKMFEVD MKIAAMHVKR KQLHQLLPNH VLQKKKKHST EGVKLTALND SSLDLSMDSD NSMSVPSPTS ATKTSPLNSS GSSQGRNSPA PAVTAASVTN IQATEVSVPQ VNSSESSGGT SSESIPQTAT QPAISPPPKP TVSRVVSSTR LVNPPPRSSG NAATSGNAAT KIPTPIVGVK RTSSPHKEES PKKTKTEEDE TSEDANCLAL SGHDKTEAKE QLDTETSTTQ SETIQTAASL LASQKTSSTD LSDIPALPAN PIPVIKNSIK LRLNR
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分子量
82.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PAPOLA (Poly-A Polymerase Alpha) is a critical enzyme involved in the post-transcriptional modification of RNA, specifically in the addition of polyadenylate (poly(A)) tails to messenger RNA (mRNA). This process is essential for mRNA stability, nuclear export, and translation efficiency, thus playing a vital role in gene expression regulation. Research on PAPOLA has gained momentum due to its implications in various biological processes and diseases, including cancer and neurodegenerative disorders, where aberrant polyadenylation may contribute to altered gene expression profiles. Furthermore, the study of PAPOLA has expanded into the realm of synthetic biology and biotechnology, where engineered variants of this protein are being explored for their potential applications in mRNA therapeutics and vaccines. By understanding the structure-function relationship of PAPOLA and its interaction with other cellular factors, scientists aim to elucidate its precise role in cellular processes and develop innovative strategies for therapeutic interventions. This research is poised to contribute significantly to the fields of molecular biology and medicine, offering potential pathways for advancing treatment options for conditions associated with dysregulated gene expression.
