Analytical Data
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Gene name
PAPOLB
- Application
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Alternative Names
PAPOLB; PAPTPoly(A) polymerase beta; PAP-beta; EC 2.7.7.19; Polynucleotide adenylyltransferase beta; Testis-specific poly(A) polymerase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NRJ5
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Expression Region
1-636 aa
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AA Sequence
MPFPVTTQGP PQPAPPPNRY GVSSPISLAV PKETDCLLTQ RLIETLRPFG VFEEEEELQR RILVLEKLNN LVKEWIREIS ESKSLPQSVI ENVGGKIFTF GSYRLGVHTK GADIDALCVA PSHVDRSDFF TSFYAKLKLQ EEVKDLRAVE EAFVPVIKLC FDGIEIDILF ARLALQTIPE DLDLRDDSLL KNLDIRCIRS LNGCRVTDEI LHLVPNIDNF RLTLRAIKLW AKCHNIYSNI LGFLGGVSWA MLVARTCQLY PNAVASTLVR KFFLVFSEWE WPNPVLLKEP EERNLNLPVW DPRVNPSDRY HLMPIITPAY PQQNSTYNVS ISTRMVMIEE FKQGLAITHE ILLSKAEWSK LFEAPSFFQK YKHYIVLLAS ASTEKQHLEW VGLVESKIRI LVGSLEKNEF ITLAHVNPQS FPAPKENPDM EEFRTMWVIG LGLKKPDNSE ILSIDLTYDI QSFTDTVYRQ AVNSKMFEMG MKITAMHLRR KELHQLLPHH VLQDKKAHST EGRRLTDLND SSFDLSAGCE NSMSVPSSTS TMKTGPLISS SQGRNSPALA VMTASVANIQ ATEFSLQQVN TNESSGVALN ESIPHAVSQP AISPSPKAMV ARVVSSTCLI SHPDLQETQQ QTYLIL
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Molecular Weight
71.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PAPOLB, or poly(A) polymerase beta, is an enzyme that plays a pivotal role in RNA metabolism, particularly in the post-transcriptional modification of RNA molecules. Research into PAPOLB has gained significance due to its involvement in various cellular processes, including mRNA stability, polyadenylation, and gene expression regulation. Abnormalities in PAPOLB function have been linked to several diseases, including cancer and neurodegenerative disorders, highlighting its potential as a therapeutic target. The enzyme's dual function—supporting both polyadenylation and poly(A) tail remodeling—has drawn attention to its mechanistic pathways and regulatory networks. Furthermore, recent advances in proteomics and molecular biology have facilitated deeper investigations into PAPOLB's structure-function relationships, offering insights into its role in RNA processing and cellular responses to stress. Understanding PAPOLB's intricate functions and interactions within the cellular milieu is crucial for developing innovative strategies to manipulate its activity for therapeutic interventions, making it a prominent focus in the fields of molecular biology and biotechnology.
