Analytical Data
-
Gene name
ARHGEF25
- Application
-
Alternative Names
ARHGEF25; ARHGP_HUMAN; GEFT ; Guanine nucleotide exchange factor GEFT
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q86VW2
-
Expression Region
1-580aa
-
AA Sequence
MRGGHKGGRC ACPRVIRKVL AKCGCCFARG GRESYSIAGS EGSISASAAS GLAAPSGPSS GLSSGPCSPG PPGPVSGLRR WLDHSKHCLS VETEADSGQA GPYENWMLEP ALATGEELPE LTLLTTLLEG PGDKTQPPEE ETLSQAPESE EEQKKKALER SMYVLSELVE TEKMYVDDLG QIVEGYMATM AAQGVPESLR GRDRIVFGNI QQIYEWHRDY FLQELQRCLK DPDWLAQLFI KHERRLHMYV VYCQNKPKSE HVVSEFGDSY FEELRQQLGH RLQLNDLLIK PVQRIMKYQL LLKDFLKYYN RAGMDTADLE QAVEVMCFVP KRCNDMMTLG RLRGFEGKLT AQGKLLGQDT FWVTEPEAGG LLSSRGRERR VFLFEQIIIF SEALGGGVRG GTQPGYVYKN SIKVSCLGLE GNLQGDPCRF ALTSRGPEGG IQRYVLQAAD PAISQAWIKH VAQILESQRD FLNALQSPIE YQRRESQTNS LGRPRGPGVG SPGRIQLGDQ AQGSTHTPIN GSLPSLLLSP KGEVARALLP LDKQALGDIP QAPHDSPPVS PTPKTPPCQA RLAKLDEDEL
-
Molecular Weight
63 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ARHGEF25, a member of the Rho guanine nucleotide exchange factor (GEF) family, has garnered significant attention in recent years due to its pivotal role in regulating Rho GTPases, which are essential for various cellular processes including cytoskeletal organization, cell migration, and proliferation. Research has indicated that ARHGEF25 may be implicated in several pathological conditions, including cancer metastasis and cardiovascular diseases, as its modulation can influence cell behavior and signaling pathways. Studies have shown that ARHGEF25 can interact with various signaling molecules, thus affecting the actin cytoskeleton and contributing to the dynamic changes in cell shape and movement. Given its potential as a therapeutic target, the development and characterization of recombinant ARHGEF25 protein are crucial for elucidating its function and role in disease. By producing ARHGEF25 as a recombinant protein, researchers can conduct detailed biochemical and structural analyses, enhancing our understanding of its mechanisms of action and interactions with other cellular components. Furthermore, this research could facilitate the identification of novel drug targets and treatment strategies for diseases where ARHGEF25 is dysregulated. Overall, the study of recombinant ARHGEF25 protein offers promising insights into its biological significance and potential therapeutic applications, emphasizing the need for further exploration in the context of cell signaling and disease modulation.
