Analytical Data
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Gene name
KPNa4
- Application
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Alternative Names
KPNa4；QIP1；Importin subunit alpha-3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O00629
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Expression Region
1-521aa
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AA Sequence
MADNEKLDNQRLKNFKNKGRDLETMRRQRNEVVVELRKNKRDEHLLKRRN VPHEDICEDSDIDGDYRVQNTSLEAIVQNASSDNQGIQLSAVQAARKLLS SDRNPPIDDLIKSGILPILVHCLERDDNPSLQFEAAWALTNIASGTSEQT QAVVQSNAVPLFLRLLHSPHQNVCEQAVWALGNIIGDGPQCRDYVISLGV VKPLLSFISPSIPITFLRNVTWVMVNLCRHKDPPPPMETIQEILPALCVL IHHTDVNILVDTVWALSYLTDAGNEQIQMVIDSGIVPHLVPLLSHQEVKV QTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHPKEKINKEAVWFL SNITAGNQQQVQAVIDANLVPMIIHLLDKGDFGTQKEAAWAISNLTISGR KDQVAYLIQQNVIPPFCNLLTVKDAQVVQVVLDGLSNILKMAEDEAETIG NLIEECGGLEKIEQLQNHENEDIYKLAYEIIDQFFSSDDIDEDPSLVPEA IQGGTFGFNSSANVPTEGFQF
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Molecular Weight
83 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KPNa4, a member of the Karyopherin family, plays a vital role in nucleocytoplasmic transport, facilitating the translocation of proteins between the nucleus and cytoplasm. Recent studies have highlighted its involvement in various cellular processes, including gene expression regulation and cell cycle progression, underscoring its significance in both normal physiological functions and pathological conditions. Research has shown that KPNa4 can interact with both nuclear localization signals (NLS) and importin-β, suggesting its dual role in mediating protein import. Furthermore, its dysregulation has been implicated in various diseases, including cancer and neurodegenerative disorders, prompting increased interest in understanding its structure and function. Notably, KPNa4 has been identified as a potential therapeutic target, leading to investigations into its role as a biomarker and in drug development. By characterizing the recombinant KPNa4 protein, researchers aim to unravel the mechanistic pathways underlying its function, which could pave the way for novel therapeutic strategies targeting nucleocytoplasmic transport. As such, the study of KPNa4 not only enhances our understanding of cellular dynamics but also provides insight into potential interventions for diseases linked to its dysfunction.
