Cat: PAX2000-10086

Recombinant Human PAAF1 Protein,His

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Analytical Data

  • Gene name

    PAAF1

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    PAAF1; WDR71Proteasomal ATPase-associated factor 1; Protein G-16; WD repeat-containing protein 71

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q9BRP4

  • Expression Region

    2-392 aa

  • AA Sequence

    AAPLRIQSD WAQALRKDEG EAWLSCHPPG KPSLYGSLTC QGIGLDGIPE VTASEGFTVN EINKKSIHIS CPKENASSKF LAPYTTFSRI HTKSITCLDI SSRGGLGVSS STDGTMKIWQ ASNGELRRVL EGHVFDVNCC RFFPSGLVVL SGGMDAQLKI WSAEDASCVV TFKGHKGGIL DTAIVDRGRN VVSASRDGTA RLWDCGRSAC LGVLADCGSS INGVAVGAAD NSINLGSPEQ MPSEREVGTE AKMLLLARED KKLQCLGLQS RQLVFLFIGS DAFNCCTFLS GFLLLAGTQD GNIYQLDVRS PRAPVQVIHR SGAPVLSLLS VRDGFIASQG DGSCFIVQQD LDYVTELTGA DCDPVYKVAT WEKQIYTCCR DGLVRRYQLS DL

  • Molecular Weight

    42.1 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

PAAF1, or P53-Associated Protein 1, is a multifunctional protein that plays a significant role in various cellular processes, including cell cycle regulation, apoptosis, and DNA damage response. Research has indicated that PAAF1 can interact with the tumor suppressor protein p53, a crucial player in maintaining genomic stability and preventing tumorigenesis. The dysregulation of p53 and its associated pathways is often implicated in cancer development. Therefore, understanding the biochemical properties and functional mechanisms of PAAF1 is vital for elucidating its role in cellular homeostasis and pathology. Recent studies have focused on recombinant expression of PAAF1 to facilitate in-depth analyses of its structure and interactions with other cellular components. The production of PAAF1 as a recombinant protein allows researchers to investigate its functional domains, post-translational modifications, and its interaction with p53 and other partners, providing insights into its contribution to tumor biology. Given the potential of PAAF1 as a therapeutic target, ongoing research aims to explore its role in cancer progression and treatment resistance, making it a key focus in the context of cancer biology and novel therapeutic strategies.

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