Analytical Data
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基因名
RBX1
- Application
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别名
RBX1；RNF75；ROC1；E3 ubiquitin-Protein ligase RBX1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P62877
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表达区间
1-108aa
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氨基酸序列
MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH
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分子量
43.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RBX1, or Ring Box 1, is an E3 ubiquitin ligase that plays a crucial role in the ubiquitin-proteasome system, which regulates protein degradation and maintains cellular homeostasis. It facilitates the transfer of ubiquitin moieties to target proteins, marking them for degradation, thus influencing various cellular processes including cell cycle progression, DNA repair, and response to stress. Abnormalities in RBX1 expression and function have been implicated in several diseases, including cancer, where it can contribute to tumor progression by altering the degradation of oncogenic and tumor suppressor proteins. Research on RBX1 recombinant protein focuses on understanding its structure-function relationship and the molecular mechanisms underlying its role in ubiquitination. This knowledge is essential for the development of therapeutic interventions that could target aberrant RBX1 activity in disease contexts. Additionally, studying RBX1 in vitro through recombinant protein expression allows for the exploration of its interactions with specific substrates and regulators, providing insights into ubiquitin-mediated signaling pathways. Overall, RBX1 remains a promising target for further research in both basic biology and potential clinical applications.
