Analytical Data
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基因名
APLF
- Application
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别名
AI452191; Aplf; APLF_HUMAN; Aprataxin and PNK-like factor
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8IW19
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表达区间
1-511aa
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氨基酸序列
MSGGFELQPR DGGPRVALAP GETVIGRGPL LGITDKRVSR RHAILEVAGG QLRIKPIHTN PCFYQSSEKS QLLPLKPNLW CYLNPGDSFS LLVDKYIFRI LSIPSEVEMQ CTLRNSQVLD EDNILNETPK SPVINLPHET TGASQLEGST EIAKTQMTPT NSVSFLGENR DCNKQQPILA ERKRILPTWM LAEHLSDQNL SVPAISGGNV IQGSGKEEIC KDKSQLNTTQ QGRRQLISSG SSENTSAEQD TGEECKNTDQ EESTISSKEM PQSFSAITLS NTEMNNIKTN AQRNKLPIEE LGKVSKHKIA TKRTPHKEDE AMSCSENCSS AQGDSLQDES QGSHSESSSN PSNPETLHAK ATDSVLQGSE GNKVKRTSCM YGANCYRKNP VHFQHFSHPG DSDYGGVQIV GQDETDDRPE CPYGPSCYRK NPQHKIEYRH NTLPVRNVLD EDNDNVGQPN EYDLNDSFLD DEEEDYEPTD EDSDWEPGKE DEEKEDVEEL LKEAKRFMKR K
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分子量
56.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
APLF (APurinic/Apyrimidinic Endonuclease Family Member 1) is a DNA repair protein that plays a crucial role in maintaining genomic stability by participating in the base excision repair (BER) pathway. Research on APLF has gained attention due to its involvement in repairing DNA damage caused by various stressors, including oxidative stress and ionizing radiation, which can lead to the formation of apurinic/apyrimidinic (AP) sites. These sites, if left unrepaired, can result in mutations, genomic instability, and contribute to the development of cancer. Moreover, APLF has been shown to interact with other repair proteins, such as PARP1 (Poly(ADP-ribose) polymerase 1), indicating its significance in DNA damage response networks. Studies have revealed that APLF is not only essential for the efficient repair of DNA but also plays a role in the cellular response to DNA damage signaling. The exploration of APLF’s mechanisms and interactions has opened up new avenues for understanding how cells combat genomic insults and maintain integrity. Consequently, APLF is being investigated as a potential target for therapeutic strategies in cancer treatment, particularly in cancers characterized by defective DNA repair pathways, offering hope for more effective interventions against these malignancies.
