Analytical Data
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基因名
AKAP1
- Application
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别名
AKAP1；AKAP149；PRKA1；A-kinase anchor Protein 1. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q92667
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表达区间
1-903aa
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氨基酸序列
MAIQFRSLFPLALPGMLALLGWWWFFSRKKGHVSSHDEQQVEAGAVQLRADPAIKEPLPVEDVCPKVVSTPPSVTEPPEKELSTVSKLPAEPPALLQTHPPCRRSESSGILPNTTDMRLRPGTRRDDSTKLELALTGGEAKSIPLECPLSSPKGVLFSSKSAEVCKQDSPFSRVPRKVQPGYPVVPAEKRSSGERARETGGAEGTGDAVLGEKVLEEALLSREHVLELENSKGPSLASLEGEEDKGKSSSSQVVGPVQEEEYVAEKLPSRFIESAHTELAKDDAAPAPPVADAKAQDRGVEGELGNEESLDRNEEGLDRNEEGLDRNEESLDRNEEGLDRNEEIKRAAFQIISQVISEATEQVLATTVGKVAGRVCQASQLQGQKEESCVPVHQKTVLGPDTAEPATAEAAVAPPDAGLPLPGLPAEGSPPPKTYVSCLKSLLSSPTKDSKPNISAHHISLASCLALTTPSEELPDRAGILVEDATCVTCMSDSSQSVPLVASPGHCSDSFSTSGLEDSCTETSSSPRDKAITPPLPESTVPFSNGVLKGELSDLGAEDGWTMDAEADHSGGSDRNSMDSVDSCCSLKKTESFQNAQAGSNPKKVDLIIWEIEVPKHLVGRLIGKQGRYVSFLKQTSGAKIYISTLPYTQSVQICHIEGSQHHVDKALNLIGKKFKELNLTNIYAPPLPSLALPSLPMTSWLMLPDGITVEVIVVNQVNAGHLFVQQHTHPTFHALRSLDQQMYLCYSQPGIPTLPTPVEITVICAAPGADGAWWRAQVVASYEETNEVEIRYVDYGGYKRVKVDVLRQIRSDFVTLPFQGAEVLLDSVMPLSDDDQFSPEADAAMSEMTGNTALLAQVTSYSPTGLPLIQLWSVVGDEVVLINRSLVERGLAQWVDSYYTSL
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分子量
97.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AKAP1 (A-kinase anchoring protein 1) is a critical scaffold protein that plays a pivotal role in signaling networks within cells by anchoring protein kinase A (PKA) and other signaling molecules to specific cellular locations, thereby influencing various physiological processes. The importance of AKAP1 in cellular signaling and its involvement in numerous pathophysiological conditions, including cancer, heart disease, and neurological disorders, has prompted significant interest in understanding its function and interactions. Research into AKAP1 has revealed that it operates not only as a spatial organizer of signaling complexes but also as a regulator of mitochondrial function and apoptosis. Recent studies have indicated that aberrations in AKAP1 expression and function could be linked to the progression of various diseases, highlighting its potential as a therapeutic target. As a result, the recombinant expression of AKAP1 has become a focal area of research, enabling scientists to explore its structural characteristics and functional roles in intricate signaling pathways. By generating recombinant AKAP1 proteins, researchers are equipped to perform detailed biochemical and biophysical analyses, allowing for a deeper understanding of its interactions with PKA and other proteins. This knowledge is crucial for developing targeted therapies that can modulate AKAP1-mediated signaling processes in disease contexts. Overall, the investigation of AKAP1 and its recombinant protein forms serves as a promising avenue for elucidating the complexities of cellular signaling and for identifying novel intervention strategies in human health and disease.
