Analytical Data
-
Gene name
PARS2
- Application
-
Alternative Names
PARS2；Probable proline--tRNA ligase. mitochondrial
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q7L3T8
-
Expression Region
30-475aa
-
AA Sequence
H HCAPRRGRRL LLSRVFQPQN LREDRVLSLQ DKSDDLTCKS QRLMLQVGLI YPASPGCYHL LPYTVRAMEK LVRVIDQEMQ AIGGQKVNMP SLSPAELWQA TNRWDLMGKE LLRLRDRHGK EYCLGPTHEE AITALIASQK KLSYKQLPFL LYQVTRKFRD EPRPRFGLLR GREFYMKDMY TFDSSPEAAQ QTYSLVCDAY CSLFNKLGLP FVKVQADVGT IGGTVSHEFQ LPVDIGEDRL AICPRCSFSA NMETLDLSQM NCPACQGPLT KTKGIEVGHT FYLGTKYSSI FNAQFTNVCG KPTLAEMGCY GLGVTRILAA AIEVLSTEDC VRWPSLLAPY QACLIPPKKG SKEQAASELI GQLYDHITEA VPQLHGEVLL DDRTHLTIGN RLKDANKFGY PFVIIAGKRA LEDPAHFEVW CQNTGEVAFL TKDGVMDLLT PVQTV
-
Molecular Weight
53.2 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PARS2, also known as mitochondrial phenylalanine-tRNA synthetase 2, is an essential enzyme involved in mitochondrial protein synthesis. It plays a critical role in the proper functioning of mitochondria by catalyzing the attachment of phenylalanine to its corresponding tRNA, a vital step in protein translation within the organelle. Research into PARS2 has gained prominence due to its potential implications in various mitochondrial disorders, which are often associated with genetic mutations affecting tRNA synthetases. These disorders can lead to diverse clinical manifestations, including neurodegenerative diseases and metabolic syndromes. Understanding the structure and function of PARS2 can provide insights into its role in mitochondrial dynamics and its involvement in disease mechanisms. Furthermore, the recombinant expression of PARS2 allows researchers to study its enzymatic properties, interaction with other mitochondrial components, and regulatory mechanisms, ultimately contributing to the development of potential therapeutic strategies. With advancements in biotechnological techniques, characterizing PARS2 through recombinant protein production can enhance our knowledge of mitochondrial biology and its pathophysiological implications, paving the way for innovative interventions in mitochondrial diseases.
