Analytical Data
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Gene name
AKAP10
- Application
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Alternative Names
AKAP10A-kinase anchor Protein 10; mitochondrial; AKAP-10
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O43572
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Expression Region
29-662aa
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AA Sequence
KV KGKEQEKTSD VKSIKASISV HSPQKSTKNH ALLEAAGPSH VAINAISANM DSFSSSRTAT LKKQPSHMEA AHFGDLGRSC LDYQTQETKS SLSKTLEQVL HDTIVLPYFI QFMELRRMEH LVKFWLEAES FHSTTWSRIR AHSLNTVKQS SLAEPVSPSK KHETTASFLT DSLDKRLEDS GSAQLFMTHS EGIDLNNRTN STQNHLLLSQ ECDSAHSLRL EMARAGTHQV SMETQESSST LTVASRNSPA SPLKELSGKL MKSIEQDAVN TFTKYISPDA AKPIPITEAM RNDIIARICG EDGQVDPNCF VLAQSIVFSA MEQEHFSEFL RSHHFCKYQI EVLTSGTVYL ADILFCESAL FYFSEYMEKE DAVNILQFWL AADNFQSQLA AKKGQYDGQE AQNDAMILYD KYFSLQATHP LGFDDVVRLE IESNICREGG PLPNCFTTPL RQAWTTMEKV FLPGFLSSNL YYKYLNDLIH SVRGDEFLGG NVSLTAPGSV GPPDESHPGS SDSSASQSSV KKASIKILKN FDEAIIVDAA SLDPESLYQR TYAGKMTFGR VSDLGQFIRE SEPEPDVRKS KGSMFSQAMK KWVQGNTDEA QEELAWKIAK MIVSDIMQQA QYDQPLEKST KL
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Molecular Weight
73.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
AKAP10, a member of the A-kinase anchoring protein (AKAP) family, plays a critical role in cellular signaling by anchoring protein kinase A (PKA) to specific subcellular compartments, thereby influencing various physiological processes. It is primarily localized in the mitochondria and has been implicated in regulating mitochondrial function, energy metabolism, and apoptosis. Dysregulation of AKAP10 expression has been associated with various diseases, including cancer and cardiovascular disorders. Research on AKAP10 recombinant protein focuses on understanding its structural characteristics and functional interactions with PKA and other signaling molecules. By producing recombinant AKAP10, scientists aim to elucidate its role in mitochondrial dynamics and explore potential therapeutic interventions. The availability of purified AKAP10 protein is essential for conducting biochemical assays, including binding studies and functional assays, which can shed light on its contribution to health and disease. Additionally, investigating the molecular mechanisms by which AKAP10 modulates signaling pathways may reveal novel targets for drug development and therapeutic strategies. Overall, the study of AKAP10 recombinant protein is vital for advancing our understanding of mitochondrial biology and its implications in human health.
