Analytical Data
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Gene name
AFAP1L1
- Application
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Alternative Names
Actin filament-associated Protein 1-like 1; AF1L1_HUMAN; AFAP1-like Protein 1; Afap1l1; AI173486; RGD1311580
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8TED9
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Expression Region
1-768aa
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AA Sequence
MDRGQVLEQL LPELTGLLSL LDHEYLSDTT LEKKMAVASI LQSLQPLPAK EVSYLYVNTA DLHSGPSFVE SLFEEFDCDL SDLRDMPEDD GEPSKGASPE LAKSPRLRNA ADLPPPLPNK PPPEDYYEEA LPLGPGKSPE YISSHNGCSP SHSIVDGYYE DADSSYPATR VNGELKSSYN DSDAMSSSYE SYDEEEEEGK SPQPRHQWPS EEASMHLVRE CRICAFLLRK KRFGQWAKQL TVIREDQLLC YKSSKDRQPH LRLALDTCSI IYVPKDSRHK RHELRFTQGA TEVLVLALQS REQAEEWLKV IREVSKPVGG AEGVEVPRSP VLLCKLDLDK RLSQEKQTSD SDSVGVGDNC STLGRRETCD HGKGKKSSLA ELKGSMSRAA GRKITRIIGF SKKKTLADDL QTSSTEEEVP CCGYLNVLVN QGWKERWCRL KCNTLYFHKD HMDLRTHVNA IALQGCEVAP GFGPRHPFAF RILRNRQEVA ILEASCSEDM GRWLGLLLVE MGSRVTPEAL HYDYVDVETL TSIVSAGRNS FLYARSCQNQ WPEPRVYDDV PYEKMQDEEP ERPTGAQVKR HASSCSEKSH RVDPQVKVKR HASSANQYKY GKNRAEEDAR RYLVEKEKLE KEKETIRTEL IALRQEKREL KEAIRSSPGA KLKALEEAVA TLEAQCRAKE ERRIDLELKL VAVKERLQQS LAGGPALGLS VSSKPKSGET ANKPQNSVPE QPLPVNCVSE LRKRSPSIVA SNQGRVLQKA KEWEMKKT
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Molecular Weight
86.4 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
AFAP1L1, or actin filament-associated protein 1-like 1, has emerged as a significant subject of research due to its potential role in various cellular processes, including cytoskeletal organization and signal transduction. As a member of the AFAP family, AFAP1L1 is involved in regulating actin dynamics, which are crucial for cell motility, shape maintenance, and division. Studies have shown that alterations in the expression or function of AFAP1L1 are linked to several pathologies, including cancer progression and metastasis. Consequently, understanding the molecular mechanisms underlying AFAP1L1 function and its interaction with other proteins may provide insights into its contributions to disease states. The recombinant expression of AFAP1L1 is vital for these studies, allowing researchers to analyze its biochemical properties and functional roles in vitro and in vivo. By employing techniques such as protein purification and characterization, scientists can elucidate the complex pathways in which AFAP1L1 is involved, potentially leading to the identification of novel therapeutic targets. Given the interplay between actin dynamics and multiple cellular processes, continued exploration of AFAP1L1 through recombinant protein studies is essential to uncover its full biological relevance.
