Analytical Data
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基因名
SPI1
- Application
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别名
SPI1;Transcription factor PU.1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P17947
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表达区间
1-270aa
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氨基酸序列
MLQACKMEGFPLVPPPSEDLVPYDTDLYQRQTHEYYPYLSSDGESHSDHYWDFHPHHVHSEFESFAENNFTELQSVQPPQLQQLYRHMELEQMHVLDTPMVPPHPSLGHQVSYLPRMCLQYPSLSPAQPSSDEEEGERQSPPLEVSDGEADGLEPGPGLLPGETGSKKKIRLYQFLLDLLRSGDMKDSIWWVDKDKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGGLAERRHPPH
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分子量
35.1kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of SPI1 recombinant proteins is essential due to their significant role in various biological functions, particularly in immune response and hematopoiesis. SPI1, also known as PU.1, is a transcription factor that is critical for the development of blood cells and is involved in the regulation of gene expression in myeloid and lymphoid lineages. Understanding its mechanisms can shed light on the pathogenesis of several conditions, including leukemia and other hematological disorders. The production of SPI1 recombinant proteins allows researchers to examine its structure and function, facilitating studies on its role in transcriptional regulation, interactions with other proteins, and its impact on cellular processes. Investigating SPI1 in different contexts, including its potential as a therapeutic target in cancers, can lead to advancements in treatment strategies. Moreover, the use of recombinant techniques enables the generation of specific mutations or fusion proteins, providing deeper insights into the functional domains of SPI1. This research is not only pivotal for basic science but also has implications for the development of novel clinical applications in the field of oncology and regenerative medicine. By characterizing the biochemical properties and biological activities of SPI1 recombinant proteins, researchers aim to elucidate their contribution to normal and aberrant cell functions, paving the way for innovative approaches to combat blood-related diseases.












